一种轮状病毒特异抗体ELISA检测方法  

An ELISA Method for Rotavirus Specific Antibody Detection

作　　者：刘馨[1] 孙茂盛[1] 

机构地区：[1]中国医学科学院中国协和医科大学医学生物学研究所,云南昆明650118

出　　处：《动物医学进展》2005年第3期96-98,共3页Progress In Veterinary Medicine

摘　　要：在实验室条件下,建立较为快速的轮状病毒抗体 ELISA 检测方法。利用组织培养的轮状病毒 SA11 株经初步的浓缩和纯化后,作为包被抗原包被酶标板。SDS PAGE分析表明,经浓缩纯化后,轮状病毒的主要抗原均存在。将测试血清用SA11中和后,轮状病毒或其抗原免疫血清(肠黏液,粪便悬液)的OD490值比中和前有明显的下降,下降比率大于45%,而对照组血清的OD490值与中和前相比没有明显的变化,下降比率小于 9%。表明用此 ELISA 体系检测轮状病毒抗体具有特异性。A rapid ELISA method for Rotavirus antibody detection was established under the common laboratory conditions. Cell-cultured Rotavirus SA11 was concentrated and partial purified and then used to coat the 96-well microtiter plates. The virus concentration was analyzed by SDS-PAGE to confirm the existence of most virus antigens. The tested sera, intestinal fluids, and fecal supernatant were neutralized by SA11. After neutralization, the OD_(490) values of the samples collected from rotavirus or its antigens immunized animals decreased significantly(>45% of the original specimens),while the OD_(490) values of the control samples did not change obviously ( <9% of the original specimens).The result demonstrated that the ELISA method was specific for rotavirus antibody detection.

关 键 词：轮状病毒 酶联免疫吸附试验 中和实验 抗血清 

分 类 号：S854.43[农业科学—临床兽医学]