运动对2型糖尿病大鼠胰岛细胞形态与功能的影响  

Effect of exercise on the morphology and function of pancreatic beta-cell in type 2 diabetes rats model

作　　者：谷成英[1] 袁莉[1] 唐兆生[1] 刘赟[1] 祝炼[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院内分泌科,武汉430022

出　　处：《中华物理医学与康复杂志》2005年第3期145-148,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：教育部留学回国人员启动基金(No.2002);湖北省自然科学基金项目(No.2002AB136)

摘　　要：目的研究游泳运动对胰岛细胞形态和β细胞分泌功能的影响,探讨胰岛素抵抗和胰岛β细胞功能障碍的相关作用和细胞机制,为2型糖尿病胰岛β细胞功能的早期保护和干预策略提供实验依据。方法以小剂量链脲佐菌素(STZ)腹腔内注射Wistar大鼠结合高脂饮食建立2型糖尿病动物模型,造模后分为模型组和运动组,每组7只,未造模的8只大鼠作为对照组。运动组大鼠游泳训练8周后做胰腺组织免疫组化染色,进行形态学及量化分析,同时检测血糖、血脂及血浆胰岛素水平,并与对照组和模型组进行比较。结果运动组与模型组比较,胰岛细胞形态明显改善,胰岛素阳性表达细胞密度(PCD)明显增大,胰岛素阳性表达细胞相对容积(IRV)明显减小(均P<0.01),接近对照组水平;而β细胞内胰岛素相对浓度(IRC)无明显变化;胰岛素释放功能、胰岛素敏感指数(ISI)明显改善,体重增值(△W)、肾周脂肪/体重(F/W)、血糖和血脂均明显降低。结论运动可改善胰岛素抵抗状态,逆转2型糖尿病大鼠胰岛的形态学变化,维持或增加有效β细胞数量、改善β细胞分泌功能。Objective To study the effect of swim on morphology and secretion of beta-cells. Methods Type 2 diabetes rats model was created by intraperitoneal injection of a small dose of streptozotocin plus high caloric laboratory chow with 14 animals. Then the rats were divided into a model group and an exercise group, with each group with 7 rats. After trained for swimming for 8 weeks, the pancreatic tissues of the exercise group were stained by immunohistochemistry technique, the islet cell morphology and cell quantity were measured. Moreover, insulin release function, plasma glucose and lipid were tested. Results (1)Compared with the model group, islet cell morphology improved significantly in the exercise group,insulin-expression positive cell density was improving and insulin relative volume was cut down near to the level of control group (P<0.01), but insulin relative concentration did not change. Moreover, the function of insulin releasing and insulin sensitivity index were improving as well as △Weight, fat/weight, plasma glucose, lipid decreased significantly. Conclusion By meliorating insulin resistance, exercise can reverse the change of morphology on pancreatic islet in type 2 diabetes rats, maintain or increase effective beta-cell number, and improve secretive function of beta-cell.

关 键 词：2型糖尿病 大鼠 游泳运动 胰岛细胞 胰岛素 对照组 阳性表达 游泳训练 ISI 水平 

分 类 号：R587.1[医药卫生—内分泌] R743[医药卫生—内科学]