参附注射液对败血性休克大鼠心肌损伤的影响  

Effects of Shenfu Injection on Myocardial Injury in Rats with Septic Shock

作　　者：杨进国[1] 刘先义[1] 杜大平[1] 

机构地区：[1]武汉大学人民医院麻醉科,武汉430060

出　　处：《武汉大学学报（医学版）》2005年第2期168-171,i004,共5页Medical Journal of Wuhan University

摘　　要：目的:观察参附注射液对败血性休克大鼠心肌损伤的保护作用并探讨其可能机制。方法:Wistar大鼠随机分为假手术组(C)、休克组(S)、参附治疗组(SF),各组均为 16 只。采用改良的盲肠结扎穿孔(CLP)方法复制败血性休克模型,观察术后6 h和18 h的心肌组织病理改变;免疫组织化学技术检测心肌组织中核转录因子 κB(NF κB)、细胞间粘附分子 1(ICAM 1)的表达和分布;用ELISA法检测血浆中肿瘤坏死因子 α(TNF α)、白细胞介素 10(IL 10)的含量,连续监测动脉压及心率变化。结果:较之C组, 6 h时S组大鼠动脉压即明显下降,心率增快(P<0.01),到18 h心率、动脉压均极度降低(P<0.01),而SF组18 h才出现 MAP下降(P<0.05)。与同时点 S组比较,SF组心肌组织病理损害明显减轻;SF组CLP后心肌组织 NF κB及 ICAM 1 的阳性表达比 S组明显降低(P<0.01);SF组血浆中TNF α含量较S组明显降低(P<0.01),18 h时 IL 10 含量较 S组明显降低(P<0.01)。结论:参附注射液能抑制败血性休克后心肌组织NF κB的活化及ICAM 1、TNF α、IL 10的表达从而起到心肌保护的作用。Objective: To investigate the protective effects of Shenfu injection (SF), a traditional Chinese medicine, on myocardial injury in rats with septic shock and the possible mechanisms. Methods: 48 Wistar rats were randomly divided into 3 groups with 16 in each group: sham-operation group(C group) only underwent laparotomy; septic shock group(S group) underwent cecal ligation and punctures (CLP); and Shenfu treated group (SF group) received Shenfu injection 8 ml·kg –1 ·h –1 intravenously followed by CLP. Each group was further divided into 2 subgroups: 6 h and 18 h subgroups according to the time when blood samples were taken and the rats were sacrificed. Myocardium samples of rats’ left ventricle were extracted for observation under optical microscope. Immunohistochemical staining method of SP was used to locate the expression and distribution of NF-κB and ICAM-1 in myocardium. The plasma levels of TNF-α and IL-10 were determined by enzyme-linked immunoadsordent assay (ELISA). Right femoral artery and vein were cannulated for mean artery pressure (MAP) monitoring and fluid infusion. Results: Compared with that in C group, MAP gradually decreased and heart rate gradually increased at 6 h after CLP in S group(P<0.01). And at 18 h, both MAP and heart rate markedly decreased(P< 0.01 ). However, MAP begin to decrease at 18 h in SF group(P<0.05). There were less myocardial injuries in SF group than in S group at the same stage. SF could significantly inhibit the activation of NF-κB and the expression of ICAM-1 in myocardium and decreased the concentration of TNF-α and IL-10 in plasma after CLP, compared with that in S group(P<0.01). Conclusion: Shenfu injection could protect myocardium from the injury induced by septic shock through inhibiting the activity of NF-κB and the expression of ICAM-1, TNF-α and IL-10.

关 键 词：参附注射液 核转录因子-ΚB 细胞间粘附分子一1 肿瘤坏死因子-α 白细胞介素-10 败血性休克 心肌损伤 

分 类 号：R631.4[医药卫生—外科学]