米索前列醇对去势后大鼠骨保护素的影响  

Effect of Misoprostol on Osteoprotegerin in the Bone of Ovariectomized Rats

作　　者：郑先念[1] 陈廖斌[1] 蔡林[1] 顾洁夫[1] 

机构地区：[1]武汉大学中南医院骨科,武汉430071

出　　处：《武汉大学学报（医学版）》2005年第2期176-179,共4页Medical Journal of Wuhan University

摘　　要：目的:通过给予去势后大鼠米索前列醇,了解米索前列醇对骨密度及成骨细胞分泌骨保护素 (Osteo protegerin, OPG)的影响。方法:鼠龄90 d的Wistar雌性大鼠共 30 只,分为正常对照组(A组)、去势组(B组)、去势给米索前列醇组(C组),C组去势后第2 d予以2 mg·kg-1·d-1剂量的米索前列醇灌胃,于术后28 d对各组的骨密度进行检测,同时取左侧胫骨干骺端观察骨保护素 mRNA水平的变化。结果: 骨密度检测结果显示 A组最高,C组次之,而B组最低,A组、B组、C组之间差异有显著性(P<0.05);骨保护素3组之间差别有显著性,C组最高,B组次之,A组最低(P<0.05)。结论: 米索前列醇可能通过刺激骨保护素的表达及分泌,来抑制去势后雌性大鼠骨量的丢失。Objective: To investigated the influence of misoprostol on bone mineral density (BMD) and osteoprotegerin (OPG) in ovariectomized (OVX) rats. Methods: Thirty female Wistar rats aged 3 months were divided into three groups as Sham(A), OVX(B) and OVX+ misoprostol(C)group with 10 rats in each group. The group C treated orally 2 mg·kg -1 ·d -1 misoprostol for 28 days. All rats were sacrificed and the BMD of rats was measured by dual-energy X-ray Absorption, and reverse transcription-polymerase chain reaction (RT-PCR) examination which indicated the expression of OPG on mRNA level in tibial metaphyses. Results: The BMD of rats in group A was the highest one, while in group C was higher than that in group B, and there was a significant difference among the group A, B and C. There was a significant difference in OPG among the three groups as C>B>A in turn. Conclusion: Misoprostol can effectively prevent osteoporosis in ovariectomized rats by up-regulating OPG mRNA level and inhibiting osteoclastic bone resorption.

关 键 词：米索前列醇 骨密度 骨保护素 RT-PCR 

分 类 号：R681.1[医药卫生—骨科学]