激素对鼻息肉组织肿瘤坏死因子α、血管内皮生长因子表达的影响  

Effects of Corticosteroid on Expression of TNF-α and VEGF in Nasal Polyps

作　　者：肖伯奎[1] 陶泽璋[1] 马哲兰[1] 吴玉珍[1] 孔勇刚[1] 

机构地区：[1]武汉大学人民医院耳鼻咽喉头颈外科,武汉430060

出　　处：《武汉大学学报（医学版）》2005年第2期247-249,i001,共4页Medical Journal of Wuhan University

摘　　要：目的:探讨肿瘤坏死因子(TNF)α、血管内皮生长因子(VEGF)在鼻息肉组织中的表达,以及全身应用糖皮质激素对两种细胞因子表达水平的影响。方法:采用免疫组化SP法,检测鼻息肉组织在糖皮质激素治疗前和治疗后TNF α、VEGF的表达,运用HPIAS 1000高分辨率彩色图像分析系统分别对治疗前、后鼻息肉组织的粘膜上皮细胞、腺上皮细胞、血管内皮细胞、粘膜下浸润炎性细胞、细胞间基质进行光密度测定分析。结果: ① TNF α在鼻息肉粘膜上皮细胞、血管内皮细胞、腺上皮细胞、粘膜下浸润炎性细胞均有阳性表达;VEGF在粘膜上皮细胞、血管内皮细胞、腺上皮细胞、粘膜下浸润细胞、基质有阳性表达;②激素治疗后 TNF α、VEGF表达下降,其中 TNF α在血管内皮细胞表达下降,治疗前后有显著性差异。VEGF表达在治疗前、后无明显差异。结论:全身应用激素可能通过以下机制起到治疗鼻息肉的作用:①降低鼻息肉组织中 TNF α、VEGF的含量,影响鼻息肉形成的微环境,改变其它细胞因子的表达从而调节上皮细胞的增生、血管的生成及通透性;②降低TNF α在鼻息肉血管内皮细胞的表达,减弱粘附分子、吸附分子的作用从而减轻嗜酸性粒细胞在鼻息肉组织的浸润。Objective: To study the expression of TNF-α and VEGF in nasal polyps and the effects of corticosteroid on these cell factors. Methods: The expression of TNF-α and VEGF in nasal polyps before and after treatment with corticosteroid were detected using SP immunohistochemical methods, and the optical density of mucosal epithelia, glandular epithelium, vascular endothelia and the cells in the stroma and matrix in nasal polyps were analyzed by HPIAS-1000. Results: ① The expression of TNF-α in mucosal epithelia, vascular endothelia, glandular epithelium and cells of matrix were positive in nasal polyps. The VEGF positive cells also appeared in mucosal epithelia, vascular endothelial, glandular epithelium and the cells in the stroma. ② The expression of TNF-α were significantly reduced in nasal polyps after treatment with corticosteroid, and no significant differences of the VEGF expression were detected between pre- and post-treated group. Conclusion: Corticosteroid may change the micro-environment for nasal polyps forming and contribute to the expression of cell factors that leads to the epithelia and vessels forming by restraining the expression of TNF-α and VEGF in nasal polyps. Corticosteroid may alleviate Eosinophils infiltration in nasal polyps by down-regulation the expression of TNF-α in vascular endothelial cells and reducing the activity of RANTES and ICAM-1.

关 键 词：激素 鼻息肉 肿瘤坏死因子Α 血管内皮生长因子 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]