栉孔扇贝♀×华贵栉孔扇贝♂受精及早期胚胎发育过程的细胞学荧光显微观察  

Cytological Observation on Cross Fertilization and the Development of Early Embryos of Chlamys farreri♀×C.nobilis♂ with Fluorescent Microscope

作　　者：毕克[1] 包振民[1] 黄晓婷[1] 王珏[1] 赵洋[1] 胡景杰[1] 

机构地区：[1]中国海洋大学生命科学与技术学部,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2005年第2期283-286,共4页Periodical of Ocean University of China

基　　金：国家高技术研究发展计划项目 (2 0 0 3AA60 30 2 2 ) ;国家重点基础研究发展规划项目计划 (G1 9990 1 2 0 0 9) ;山东省优秀中青年科学家科研奖励基金 (0 1BS1 0 )资助

摘　　要：为探讨贝类杂交优势 ,利用HOECHST3 3 2 5 8对已固定样品进行染色的方法 ,连续观察了栉孔扇贝♀×华贵栉孔扇贝♂受精的细胞生物学过程 ,初步证明了这 2种远缘扇贝种类之间进行杂交的可行性。结果表明 ,栉孔扇贝的成熟未受精卵子处于第一次减数分裂中前期 ,部分来自华贵栉孔扇贝的异源精子可顺利进入卵子并激发后者完成 2次减数分裂 ,排出第一及第二极体。成熟的雌雄原核形成后完成融合过程 ,形成合子核 ,受精过程结束。大部分杂交受精卵及杂种早期胚胎可以正常发育 ,但其发育进程较种内近交对照组有明显的差别 ,具体表现在延缓性和不同步性 2个方面。The cytology of the cross fertilization and the development of early embryos of Chlamys farreri♀×C.nobilis♂ were investigated under fluorescent microscopic observation with HOECHST33258 stained. The results showed that the mature eggs of C.farreri were at prophase or metaphase of meiosisⅠ. Parts of sperms from C.nobilis could bind and penetrate into the eggs from C.farreri. With the activation of these sperms, the eggs resumed their meiotic division and released their first and second polar bodies successively. The male and female pronucleus fused finally. Most hybrids could develop normally. However, distinct stunt and asynchrony characterized the development of the hybrids, compared with the control groups. This study primarily testified the feasibility of crossbreeding between the two interspecific scallops.

关 键 词：栉孔扇贝 华贵栉孔扇贝 受精 早期胚胎 发育 荧光显微观察 

分 类 号：Q813.1[生物学—生物工程] Q321.2