白细胞介素5和核因子κB在变应性鼻炎动物模型骨髓中的表达  

The expression of IL-5 and NF- κ B in the bone marrow of the model of allergicrhinitis

作　　者：韩德民[1] 王向东[1] 周兵[1] 张罗[1] 刘仲燕[1] 鞠淑英[2] 范尔钟[1] 

机构地区：[1]北京耳鼻咽喉头颈外科中心 [2]首都医科大学附属北京同仁医院检验科,北京100730

出　　处：《中国耳鼻咽喉头颈外科》2005年第2期103-105,共3页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家十五科技攻关计划(2003BA712 A11--17)国家自然科学基金资助项目(30400495)

摘　　要：目的探讨白细胞介素5(interleukin-5,IL-5)和核因子κB(nuclear factor kappaB,NF-κB)在变应性鼻炎动物模型骨髓中表达的意义。方法采用6-8周雄性SD大鼠,随机分成实验组10只和对照组10只,以卵清蛋白致敏激发制成变应性鼻炎模型,免疫组化染色检测骨髓中IL-5和NF-κB的表达。结果大鼠变应性鼻炎模型骨髓涂片中IL-5阳性细胞和NF-κB比例都显著高于对照组(P<0.05),从形态上判断IL-5阳性细胞和NF-κB阳性细胞是嗜酸性粒细胞,IL-5阳性细胞比例和NF-κB阳性细胞比例呈显著正相关(r=0.79,P<0.01)。结论大鼠变应性鼻炎动物模型骨髓原位嗜酸性粒细胞能产生IL-5和NF-κB,可能调节骨髓中嗜酸性粒细胞的增殖和分化。OBJECTIVE To elucidate the mechanism of bone marrow response regulated by interleukin-5 (IL-5) and nuclear factor kappa B(NF- κ B) in the model of allergic rhinitis. METHODS Twenty SD rats were randomly divided into allergic rhinitis group(AR group) and control group(Con group).The expression of IL-5 and NF- κ B were detected by immunohistochemistry.RESULTS The ratio of IL-5 positive cells to white cells in bone marrow smears of AR group was significantly higher than that of Con group ( P< 0.05 ).The ratio of NF- κ B positive cells to white cells in bone marrow smears of AR group was significantly higher than that of Con group (P< 0.05 ).Most of the IL-5 positive cells and NF-κ B positive cells were eosinophils morphologyically. The ratio of NF- κ B positive cells to white cells was significantly positively correlated with the ratio of IL-5 positive cells to white cells in bone marrow smears of AR group ( r= 0.79,P < 0.01). CONCLUSION Eosinophils express IL-5 and NF-κ B located in bone marrow of the rat model of allergic rhinitis and could regulate the proliferation and differentiation of eosinophils in bone marrow.

关 键 词：变应性鼻炎 骨髓 NF-κB IL-5 动物模型 白细胞介素5 核因子ΚB 表达 嗜酸性粒细胞 阳性细胞 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]