Effects of Jiantaiye (健胎液) on Estrogen Receptor and mRNA Expressions in Uterus of Mice with Embryo Implantation Dysfunction  

作　　者：刘艳娟 黄光英 杨明炜 龚萍 张明敏 陆付耳 

机构地区：[1]Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, (430030)

出　　处：《Chinese Journal of Integrated Traditional and Western Medicine》2005年第1期41-44,共4页中国中西医结合杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No.30171193)

摘　　要：Objective: To explore the effect of Jiantaiye (健胎液, JTY) on the expression of estrogen receptor (ER) and ER mRNA in uterus of mice with embryo implantation dysfunction. Methods: Embryo implantation dysfunction mouse models were induced with mifepristone and treated with JTY. All animals were sacrificed on day 8 of pregnancy. The endometrial ER and ER mRNA expressions were assessed by immunnohistochemical SP method and semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Results: Area ratio and absorbency of ER in the JTY treated group's gland and stroma were higher than those of the model group, quite similar to those of the normal control's, and ER mRNA expression in treated group's uterus was significantly higher than that in the models, but it was not significantly different from the normal control. Conclusion: JTY improves the endometrial development by increasing ER and ER mRNA expressions of uterus of mice with embryo implantation dysfunction.Objective: To explore the effect of Jiantaiye (健胎液, JTY) on the expression of estrogen receptor (ER) and ER mRNA in uterus of mice with embryo implantation dysfunction. Methods: Embryo implantation dysfunction mouse models were induced with mifepristone and treated with JTY. All animals were sacrificed on day 8 of pregnancy. The endometrial ER and ER mRNA expressions were assessed by immunnohistochemical SP method and semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Results: Area ratio and absorbency of ER in the JTY treated group's gland and stroma were higher than those of the model group, quite similar to those of the normal control's, and ER mRNA expression in treated group's uterus was significantly higher than that in the models, but it was not significantly different from the normal control. Conclusion: JTY improves the endometrial development by increasing ER and ER mRNA expressions of uterus of mice with embryo implantation dysfunction.

关 键 词：Jiantaiye embryo implantation estrogen receptor 

分 类 号：R285[医药卫生—中药学]