鳢肠水溶性部位的化学和药理研究  

Studies on the Chemical Constituents from Hydrophilic Fractions of Eclipta Prostrata L. and Their Pharmacological Effects

作　　者：杨韵若[1] 聂宝明[1] 邓克敏[1] 陈泽乃[1] 陆阳[1] 

机构地区：[1]上海第二医科大学基础医学院化学教研室,上海200025

出　　处：《上海第二医科大学学报》2005年第3期223-226,231,共5页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市卫生局中医药科研基金(2002Y004)上海第二医科大学自然科学基金(03XY21013)资助项目

摘　　要：目的研究鳢肠属植物鳢肠水溶性部位的化学成分及其药理活性。方法通过溶剂提取法和色谱技术分离化学成分,运用光谱手段进行结构鉴定,利用C6细胞和PC12细胞观察鳢肠水溶性成分对细胞增殖活性的抑制作用。结果从该部位分离得到3个三萜皂苷,分别为3-O-β-D-吡喃葡糖刺囊酸(Ⅰ)、3-O-β-D-吡喃葡糖刺囊酸-28-O-β-D-吡喃葡糖苷(Ⅱ)和3-O-(2-O-硫酰基-β-D-吡喃葡糖)刺囊酸(Ⅲ),还分离得到了豆甾醇(Ⅳ)和蟛蜞菊内酯(Ⅴ);化合物Ⅰ具有抑制C6细胞和PC12细胞增殖的活性。结论化合物Ⅰ是鳢肠水溶性部位抑制细胞增殖的活性成分。Objective To investigate the chemical constituents from hydrophilic fractions of Eclipta prostrata L. and their pharmacological effects. Methods Constituents were isolated by means of extraction and chromatographic separations, identified by spectral analysis, and their bioactivity was evaluated on glioma C6 cells and PC12 cells. Results Five compounds were isolated and identified as: 3-O-β-D- glucopyranosyl-echinocystic acid (Ⅰ) , 3-O-β-D-glucopyranosyl-echinocystic acid-28-O-β-D-glucopyranoside (Ⅱ ) , 3-O-( 2-O-sulfuryl-β-D-glucopyranosyl) -echi-nocystic acid ( Ⅲ ) , stigmasterol ( Ⅳ ) and wedelolactone ( Ⅴ ) ; compound I could inhibit the proliferation of C6 cells and PC12 cells. Conclusion Compound I is the active constituent in hydrophilic fractions of E. prostrata for the inhibitory effect to cell proliferation.

关 键 词：部位 C6细胞 药理研究 PC12细胞 细胞增殖活性 葡糖苷 三萜皂苷 鳢肠 蟛蜞菊 内酯 

分 类 号：R282[医药卫生—中药学]