药蒲公英再生体系的建立和优化  

Establishment and Optimization of the Regeneration System for Common Dandelion (Taraxacum officinale Weber)

作　　者：陈华[1] 李平[1] 刘晶[1] 李银心[1] 

机构地区：[1]中国科学院植物研究所光合作用与环境分子生理学重点实验室

出　　处：《生物工程学报》2005年第2期244-249,共6页Chinese Journal of Biotechnology

基　　金：国家"8 63"高技术研究与发展计划项目资助 (No .2 0 0 3AA62 70 10 )~~

摘　　要：通过愈伤组织诱导和直接不定芽再生途径 ,建立了药蒲公英的快速高效再生系统。叶片外植体在含 0 2mg LIAA和1mg LTDZ的MS培养基中培养 2周后便产生大量的丛生芽 ,在含有 0 5mg L 2 ,4 D和 2mg L 6 BA的MS培养基中培养 30d后 ,形成明显的愈伤组织 ,愈伤组织块在含 1 0mg L 6 BA的MS培养基中成功再生。对 9株再生植株进行RAPD检测表明 ,部分植株在DNA水平上发生了变异。与对照相比 ,再生植株的主要抗氧化成分无明显变化 ,保证了有效成分的稳定。A protocol is presented for direct and indirect regeneration of common dandelion (Taraxacum officinale Weber) from leaf and petiole explants. Mult iple shoots were obtained on MS medium containing 0.2 mg/L IAA and 1 mg/L TDZ. For i ndirect regeneration, fragile calli were obtained from leaf and petiole explants on MS medium supplemented with 0.5 mg/L 2,4-D and 2.0 mg/L 6-BA. Regenerate d pl antlets were obtained when these calli were cultured on MS medium containing 1. 0 mg/L 6-BA. Random amplified polymorphic DNA (RAPD) analysis of nine regenerated plantlets revealed 61 scorable bands from 10 primers, including three specific b ands.

关 键 词：药蒲公英 愈伤组织培养 器官发生 RAPD 

分 类 号：S567.2[农业科学—中草药栽培]