青岛地区2000-2003年HFRS患者汉坦病毒部分M基因的检测及其分型研究  

Analysis of part of M gene and genotyping for M segment of hantavirus detected from HFRS patients'sera in Qingdao region during 2000-2003

作　　者：乔刚[1] 逄淑申[2] 张洪乐[2] 龚艳[1] 程茂玲[1] 李震海 

机构地区：[1]胶南市人民医院检验科,青岛266400 [2]胶南市人民医院内科,青岛266400 [3]青岛市卫生监督所

出　　处：《中华实验和临床病毒学杂志》2005年第1期22-24,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　调查山东省青岛地区 2 0 0 0 - 2 0 0 3年汉坦病毒的流行情况 ,研究病毒在该地区的分子流行病学特征。方法　采集肾综合征出血热 (HFRS)急性期患者血清标本 6 4份 ,提取血清中病毒RNA作为模板 ,根据GenBank中汉坦病毒基因序列 ,设计HTN型和SEO型的通用外套引物 ,同时分别设计HTN和SEO型的特异性引物作为内套引物 ,RT nest PCR扩增汉坦病毒基因组M片段G1区基因并测序。利用DNAStar软件进行核苷酸序列分析。结果　在 6 4份标本中 ,用HTN型特异性引物扩增出 6份 ,占 9% ;用SEO型特异性引物扩增出 2 5份 ,占 39% ;总阳性率为 4 8%。总体看来青岛地区流行的毒株以SEO为主。SEO型汉坦病毒间核苷酸序列的差异相对较小 ,其基因的离散率为0.3%～ 8.9%。HTN型汉坦病毒的变异率较高 ,其基因的离散率为 2.6 %～ 11.2 %。结论　青岛地区是以SEO型汉坦病毒的流行为主 ,HTN型并存的混合疫区 ;Objective To study the moleculer epidemiological characteristics of hantavirus seen during 2000-2003 in Qingdao region of Shandong province. Methods Sera were collected from 64 patients with hemorrhagic fever with renal syndrome (HFRS) and viral RNA was extracted from the sera. HTN and SEO universal primers were designed as outer primers and HTN and SEO specific primers as inner primers. G1 gene region of M segment from hantavirus was amplified by using RT-nest-PCR for sequencing. The data of nucleotide sequences were analyzed by DNA star software. Results Six cases were positive by HTN specific primer of total cases (9%);25 of 64 cases by SEO specific primer(39%);total positive rate was 48%. In general, SEO type was a prevalent type of hantavirus in Qingdao region. The variation of the nucleotide sequences among SEO viruses (nucleotide sequence divergence ranged from 0.3%～8.9%) was lower than that among HTN type (nucleotide sequence divergence ranged from 2.6%～11.2%). Conclusion Majority of hantavirus found in Qingdao region belonged to SEO type and still a few strains belonged to HTN type. Most of the HTN viruses were detected in Jiaonan county.

关 键 词：SEO型汉坦病毒 HFRS 患者 青岛地区 分型 M基因 扩增 特异性引物 毒株 疫区 

分 类 号：R446.5[医药卫生—诊断学]