登革病毒Ⅱ型NS1基因在小鼠体内诱导的DNA免疫  

Inoculation of plasmid expressing the dengue 2 NS1 gene elicits immunity in mice

作　　者：杨春雨[1] 陈元[1] 晏辉钧[1] 方丹云[1] 薛耀华[1] 江丽芳[1] 

机构地区：[1]中山医科大学微生物教研室,广州510089

出　　处：《中华实验和临床病毒学杂志》2005年第1期71-73,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　研究含有登革病毒Ⅱ型NS1基因的重组质粒肌内注射小鼠后在其体内诱导的细胞和体液免疫。方法　用含有登革病毒NS1基因的真核表达质粒pCNX2 NS1于小鼠胫前肌注射并加强免疫 2次。然后定期处死 ,采集血液标本以及小鼠脾细胞 ,检测小鼠的体液和细胞免疫。结果　在末次免疫后 4周检测到小鼠抗NS1抗体 ,并且检测到小鼠CD4+ 、CD8+ 亚群的变化。结论　含有登革病毒NS1基因的真核表达质粒pCNX2-NS1免疫小鼠后 ,可以诱导小鼠产生针对NS1的稳定特异性体液。Objective To study cellular and humoral immune responses to NS1 protein in mice inoculated intramuscularly with recombinant plasmid expressing dengue 2 NS1 gene. Methods The eukaryotic expressing plasmid pCNX2-NS1 was injected into tibialis anterior muscle in mice. The mice were subsequently boosted with the same dose and same method twice after the initial inoculation. The mice were killed at four-week intervals and their serum and spleen cells were harvested for further test. Results Dengue 2 antibodies were detectable in the sera from inoculated animals four weeks after the last boost. The changes of CD_4^+ T lymphocyte and CD_8^+ T lymphocyte were also determined by flow cytometry. Conclusion The recombinant plasmid containing dengue 2 NS1 genes is immunogenic in intramuscularly inoculated mice. The vaccinated mice produced dengue-2 specific and long lasting immunity.

关 键 词：免疫小鼠 登革病毒 体内 诱导 NSI DNA免疫 NS1基因 抗N 真核表达质粒 体液 

分 类 号：R392[医药卫生—免疫学]