中药心康口服液对柯萨奇B3病毒感染小鼠促干扰素诱生和保护心肌的作用观察  

Effect of Chinese herbal medicine Xin-kang oral liquid on interferon-induction and its antiviral activity in Coxsackievirus B3 infected mice

作　　者：万素君[1] 李建农[2] 赵红[3] 王丽霞[1] 黄霞珍[1] 诸雁 陈鸿珊[2] 

机构地区：[1]中国中医研究院广安门医院基础医学研究室,北京100053 [2]中国医学科学院医药生物技术研究所 [3]中国医学科学院阜外医院

出　　处：《中华实验和临床病毒学杂志》2005年第1期77-79,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金资助 (3 9770 916)

摘　　要：目的　研究中药心康口服液对柯萨奇B3病毒 (CVB3)感染小鼠体内促内源性干扰素(IFN)诱生 ,抗病毒保护心肌的作用效果 ,以探讨中药治疗的作用机制。方法　用嗜心肌株CVB3感染小鼠腹腔 ,诱发心肌炎。感染前小鼠以 30和 12g·kg 1 ·d 1 的心康口服液灌胃 2d ,病毒对照组以相同剂量的饮用水替代安慰用药 ;以外源性IFN 10 6 U·kg 1 ·d 1 皮下注射作为阳性对照组。同时设正常小鼠对照 ,不作任何处理。持续用药至感染后 5、10、和 2 0d ,取全血分离血清 ,采用细胞病变法检测血清中IFN的活性 ;剖取心脏固定后制片 ,光镜下进行心肌病理形态学检察。结果　心康口服液有促进感染小鼠体内诱生IFN的作用 ,感染期不同时间IFN诱生水平平均为 2 9 2U 0 1ml,较病毒对照组平均 12 6U 0 1ml水平有较大提高 ,且心肌病变程度与IFN诱生水平密切相关。接受外源性IFN组的小鼠 ,心肌病变程度明显比病毒对照组轻 ,但血清IFN水平低于心康口服液各组。结论　中药心康口服液能使感染CVB3产生心肌炎的小鼠体内促进IFN诱生 ,从而发挥抗病毒、保护心肌的作用效果 ;Objective To investigate the effect of Chinese herbal medicine Xin-kang oral liquid on interferon (IFN)-induction and its antiviral activity in Coxsackievirus B3 myocardiotropic virus strain (CVB3) infected mice. Methods The Xin-kang oral liquid was given orally to mice two days prior to the challenge of CVB3 virus to induce myocarditis. Two dosages of Xin-kang oral liquid crude herbal medicine 30 g·kg^(-1)·d^(-1) and 12 g·kg^(-1)·d^(-1) were given to the mice of different treatment groups respectively, sterilized water was given to the mice of virus control group. IFN-α 10~6 U·kg^(-1)·d^(-1) S.C was given to the infected mice as positive drug control group.The mice were sacrificed on 5th, 10th and 20th day of infection for evaluation, the levels of serum interferon (IFN) were titrated with vesicular stomatitis virus (VSV) and cardiac tissue was fixed and sectioned. The quantitative histological changes at various stages of myocarditis were observed. Results In the infected mice fed with 30 g·kg^(-1)·d^(-1) or 12 g·kg^(-1)·d^(-1) of Xin-kang oral liquid orally for 5,10 and 20 days, the mean titer of serum IFN of Xin-Kang oral liquid treated group was markedly higher (29.3 U/0.1 ml) than that of virus control group (12.6 U/0.1 ml). The level of serum IFN in IFN treated positive control mice was lower than that of Xin-kang treatment groups. The histological examination showed extensive myocardial necrosis and cellular infiltration in virus control group, but necrosis and cellular infiltration were less severe in Xin-kang treatment groups of mice. It is demonstrated that there were close correlation between the degree of myocardial lesions and the level of IFN-induction in treated mice. Conclusion Xin-kang oral liquid could facilitate the induction of endogenous interferon that exerted its antiviral activity in CVB3 infected mice. This can help us to understand better the mechanism of anti-CVB3 effect of Xin-Kang oral liquid.

关 键 词：IFN 小鼠 口服液 心肌 毒感染 柯萨奇B3病毒 对照组 作用效果 保护 持续用药 

分 类 号：R285[医药卫生—中药学]