骨髓间充质干细胞在受伤大鼠玻璃体内可分化为神经样细胞  

Mesenchymal stem cells differentiate into nerve-like cells in the vitreous body of injured rat

作　　者：项平[1] 黄锦桃[2] 侯春莲[2] 李海标[3] 

机构地区：[1]蚌埠医学院附属医院中心实验室,蚌埠市233004 [2]中山大学基础医学院组织胚胎学教研室 [3]中山大学基础医学院组胚教研室,510080

出　　处：《解剖学研究》2005年第1期10-11,F002,共3页Anatomy Research

基　　金：安徽省教育厅自然科学研究项目(2005KJ287);国家重点基础研究课题(973)资助项目(G199054301-2)

摘　　要：目的探讨骨髓间充质干细胞(MSCes)在受损大鼠玻璃体内分化。方法分离SD青年大鼠股骨,L鄄DMEM冲洗骨髓腔,制备细胞悬液接种培养,消化传代纯化MSCs。切断SD大鼠视神经,其近残端缝接一段自体坐骨神经,经玻璃体注射标记的MSCs。动物存活4周,免疫组化检测存活MSCs在玻璃体内是否表达波形蛋白(Vimentin)、NF、GFAP和层黏连蛋白(Laminin)。结果在受损大鼠玻璃体内存活的MSCs表达Vimentin、NF和GFAP,Laminin无表达,NF和GFAP表达率分别为6.0%±3.4%和10.0%±3.1%。结论MSCs移植受损大鼠玻璃体内,存活细胞大部分没有分化,小部分可分化为神经元样细胞和星形胶质样细胞。Objective To investigate the differentiation of mesenchymal stem cells(MSCs) in the vitreous body of injured rat. Methods Rat MSCs were separated from femur marrow by flushing out with low glucose DMEM, and planted in plastic culture flask. After optic nerve (ON) transection, an autologous sciatic nerve (attached graft, AG) was removed and sutured to the proximal stump of the ON. MSCs was injected intravitreously. Animals were allowed to survive in 4 weeks, MSCs in vitreous boby were observed by immunohistochemistry. Results The MSCs injected in vitreous boby positively expressed Vimentin, NF and GFAP, didn't expressed Laminin. The celles showed NF and GFAP were 6%±3.4%,10%±3.1%, respectively. Conclusion Mesenchymal stem cells can differentiate into neural cells and astrocyte in the vitreous body of injured rat.

关 键 词：玻璃体 体内 大鼠 NF GFAP 骨髓间充质干细胞 存活 分化 活细胞 DMEM 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R774[医药卫生—基础医学]