紫外光聚合法制备L-DBTA手性分子印迹聚合物的研究 Ⅱ.聚合物性能  

Study on the Molecular Imprinted Polymers with Recognition Properties Towards to Dibenzoyl-tartaric Acid Prepared by Photo-polymerization Method Ⅱ. Characterizing Molecular Recognition Properties

作　　者：杨座国[1] 许振良[1] 

机构地区：[1]华东理工大学化学工程研究所,上海200237

出　　处：《功能高分子学报》2005年第1期42-46,共5页Journal of Functional Polymers

基　　金：国家重点基础研究发展计划(973计划)资助(2003CB615705)

摘　　要：以丙烯酸为功能单体,二苯甲酰 L 酒石酸(L DBTA)为模板分子,三羟甲基丙烷三丙烯酸酯(TMPTA)为交联剂,采用紫外光聚合方法合成了L DBTA手性分子印迹聚合物。通过 HPLC表征,表明合成的手性分子印迹聚合物对L DBTA模板分子具有很好的识别性,L DBTA的选择性比二苯甲酰 D 酒石酸(D DBTA)高。通过Scatchard分析表明,L DBTA手性分子印迹聚合物中只存在一类影响聚合物识别能力的结合位点。293.15 K时,结合位点的平衡离解常数为0.064 mmol/L,最大表现结合容量为 6.4 mg/g。MIPs结合热力学研究表明,印迹分子 L DBTA与分子印迹聚合物手性识别基团之间的识别机理可以用Langmuir等温吸附描述,结合热力学参数为:ΔH =7.40 kJ/mol,ΔS =42.74 J/(mol·K),ΔG298 =-5.34kJ/mol。L DBTA与MIPs相互作用速率快,表观活化能为7.40 kJ/mol。Using a functional monomer(methyl methacrylate, acrylamide, ethyl acrylate or acrylic acid) and a crosslinker(TMPTA), molecularly imprinted polymers(MIPs) with recognition properties to dibenzoyl-L-tartaric acid(L-DBTA) were prepared by photopo-polymerization method in the presence of a template(L-DBTA) in acetonitrile solution. High-performance liquid chromatographic (HPLC) analysis of molecularly imprinted polymers (MIPs) of dibenzoyl-L-tartaric acid (L-DBTA) with recognition properties were conducted to evaluate the influences of various factors on DBTA binding. The experimental results indicated that the MIPs had higher L-DBTA binding ability than D-DBTA′s and also higher than corresponding non-imprinted polymers. Based on Scatchard analysis, one kind of binding sites was formed in the L-DBTA MIPs. At 293.15K, its equilibrium dissociation constant(K_d) was 0.064mmol/L while the maximum apparent binding capacity(Q_m) was 6.4mg/g. On the basis of isothermal binding thermodynamic investigation, the recognition mechanism between L-DBTA and MIP recognition group was described by Langmuir isothermal binding. Therefore, some thermodynamic parameters were obtained: ΔH=7.40kJ/mol, ΔS=42.74J/mol.K, ΔG_(298)=-5.34kJ/mol. The kinetics analysis illustrated that the interaction between L-DBTA and MIP was very fast and the apparent activation energy was 7.40kJ/mol.

关 键 词：紫外光聚合法 L-DBTA 聚合物 手性化合物 化学结构 分子印迹技术 

分 类 号：O631[理学—高分子化学]