不同方式鼓室内给药后豚鼠耳蜗外淋巴液中地塞米松代谢动力学特征  

Dexamethasone pharmacokinetics in perilymph of guinea pig after different topical administrations

作　　者：侯东明[1] 吴皓[1] 杨军[1] 张萍[1] 张顺国[2] 陈洁[1] 

机构地区：[1]上海第二医科大学附属新华医院耳鼻咽喉头颈外科,上海200092 [2]上海第二医科大学附属新华医院药剂科临床药理室

出　　处：《临床耳鼻咽喉科杂志》2005年第7期307-310,共4页Journal of Clinical Otorhinolaryngology

基　　金：上海市科委基金资助项目(No:034119819)

摘　　要：目的:分析不同方式鼓室内给药后豚鼠耳蜗外淋巴液中地塞米松(DEX)代谢动力学特征,为临床选择鼓室内给药方式提供实验依据。方法:采用浸满DEX溶液明胶海绵颗粒置于圆窗龛内和鼓室内灌注两种鼓室内给药方式,采集给药后1～6h豚鼠鼓阶外淋巴液,应用高效液相色谱仪分别检测其中的DEX浓度。结果:相同浓度(10g/L)的DEX溶液经明胶海绵圆窗龛给药后外淋巴液中的DEX浓度1h时最高,DEX生物半衰期t1/2为2.37h。鼓室内灌注给药后外淋巴液中的DEX浓度1h20min时最高,DEX生物半衰期为t1/2为2.26h。除1h20min、1h40min和2h外,两种方式鼓室内给药后外淋巴液中DEX的浓度相比差异均有统计学意义(均P<0.05)。结论:明胶海绵颗粒圆窗龛给药和鼓室内灌注给药两种方式鼓室内给药后DEX均能有效地经圆窗膜渗透进入外淋巴液,大部分时间明胶海绵颗粒圆窗龛给药外淋巴液中的DEX浓度高于鼓室内灌注给药。明胶海绵圆窗龛给药的生物半衰期较长。Objective:To determine pharmacokinetics profiles of dexamethasone (DEX) in perilymph of guinea pig with different intratympanic administrations,and to provide experimental evidence for clinical choice about administration.Method:Two different topical administrations placing a granule gelfoam soaked with DEX(10 g/L) in the niche of round window of guinea pig and infusing tympanic bulb with DEX solution(10 g/L) were conducted. Perilymph of the scala tympani was sampled 1-6 h after administration.DEX in the samples was assayed with high-performance liquid chromatograghy.Result:The concentrations of DEX in perilymph after placing a granule gelfoam soaked with DEX in the niche of round window were significantly higher than that after intratympanic infusing.The half-life time of DEX after placing a granule gelfoam was longer.Conclusion:DEX could effectively penetrate round window membrane into perilymph after different intratympanic administrations, and the method of placing a granule gelfoam soaked with DEX in the niche of round window is more effective than that of intratympanic infusing.

关 键 词：地塞米松 鼓室内给药 耳蜗水管 药物代谢动力学 

分 类 号：R764.83[医药卫生—耳鼻咽喉科]