昼夜节律对PEMFs暴露小鼠血液流变学及红细胞脆性的影响  

Effects of circadian rhythm on hemorrheology and erythrocyte fragility in PEMFs exposed mice

作　　者：申广浩[1] 罗二平[1] 路丽华[2] 吴小明[1] 徐巧玲[3] 周龙甫[1] 李丽荣[1] 刘鲁伟[4] 

机构地区：[1]第四军医大学生物医学工程系军队卫生装备与计量学教研室,陕西西安710033 [2]第四军医大学药学系化学教研室,陕西西安710033 [3]第四军医大学护理系,陕西西安710033 [4]第四军医大学门诊部,陕西西安710033

出　　处：《第四军医大学学报》2005年第6期562-564,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目(50077023)

摘　　要：目的: 探讨昼夜节律对脉冲电磁场暴露小鼠血液流变学的影响.方法: 将30只雄性小鼠随机分为3组(n=10): 对照组、磁场A组和磁场B组.两磁场组饲养于强度为8×10-4 T的脉冲电磁场(pulsed electromagnetic fields,PEMFs)环境中(f=15 Hz),暴磁时间均为6 h/d(磁场A组为白天10∶00～16∶00,磁场B组为夜间22∶00～4∶00).对照组饲养于线圈中,但不暴磁.10 d后统一摘右眼取血,对血液流变学指标及红细胞脆性进行测定.结果: 磁场组与对照组比较,血液流变学特性以及红细胞脆性的差异均有统计学意义(P<0.05);两磁场组(A,B)之间比较,血液流变学各指标和开始溶血NaCl溶液浓度的差异具有显著性意义(P<0.05),而完全溶血的浓度差异不明显(P>0.05).结论: PEMFs可以改善小鼠血液流变特性,降低红细胞脆性,白天暴磁效果优于夜间,提示昼夜节律对其生物效应有影响.AIM: To study the effects of circadian rhythm on the hemorrheology and erythrocyte fragility by exposing mice to pulsed electromagnetic fields (PEMFs).METHODS: Thirty male BALB/c mice were randomly divided into 3 groups: control,magnetic A,and magnetic B.The mice of both magnetic groups (A,B) were exposed to PEMFs(f=15 Hz) 6 h per day,with the intensity set to 8×10 -4 T,but the two magnetic groups were exposed at different time period,with daytime 10∶00-16∶00 for group A and nighttime 22∶00-4∶00 for group B.The mice of control group were fed in coils with sham exposure.After 10 d,the changes of the properties of hemorrheology and erythrocyte fragility were observed.RESULTS: Statistically significant differences were observed in the values of hemorrheology and erythrocyte fragility between the magnetic group (A or B) and control group (P<0.05).The values of hemorrheology and the concentrations of normal hemolysis in magnetic group A obviously decreased (P<0.05),compared with those of magnetic group B.But,no significant differences were found in the concentrations of complete hemolysis between the two magnetic groups (P>0.05).CONCLUSION: PEMFs are effective in improving the hemorrheology and decreasing the erythrocyte fragility.Effects of exposure in daytime is better than that in nighttime.The bio-effects of PEMFs are affected by the circadian rhythm.

关 键 词：脉冲电磁场 小鼠 血液流变学 红细胞脆性 

分 类 号：R312[医药卫生—基础医学]