低强度脉冲电磁场对大鼠成骨细胞的影响  

Effects of low-intensity pulsed electromagnetic fields on osteoblasts in rats

作　　者：李丽荣[1] 罗二平[1] 申广浩[1] 韩利华[2] 吴晓明[1] 周娅蕾[1] 周龙甫[1] 

机构地区：[1]第四军医大学生物医学工程系军队卫生装备与计量学教研室 [2]第四军医大学西京医院骨科研究所,陕西西安710033

出　　处：《第四军医大学学报》2005年第6期571-573,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目(50377044)

摘　　要：目的: 观察低强度脉冲电磁场 (pulsedelectromag neticfields,PEMFs)对体外培养成骨细胞增殖、分化、体外骨形成的影响.方法: 取出生后( <24h)的SD仔鼠 2只,骨组织块培养法分离、培养成骨细胞.频率 15Hz,场强 8Gs的PEMFs作用于成骨细胞.应用MTT方法、碱性磷酸酶测定、四环素荧光标记检测成骨细胞增殖、分化、体外骨形成情况.结果:①MTT检测结果显示对照组A490nm值为(0 330±0 020),实验组A490nm值为 ( 0 379±0 038 ),两组间有显著差异 (P<0 01).②ALP检测结果显示对照组A410nm值为 ( 0 170±0 029),实验组A410nm值为 (0 206±0 018),两组间有显著差异(P<0 01 ).③钙结节数量实验组也明显多于对照组.结论: PEMFs作用促进成骨细胞增殖、分化、加速了体外骨形成.AIM: To observe the effects of low-intensity pulsed electromagnetic fields (PEMFs) on the proliferation,differentiation and bone tissue-like formation of cultured rat osteoblasts.METHODS: Osteoblasts isolated from newly born (<24 h) SD rats were cultured by explants outgrowth technique.The osteoblasts in the PEMFs group were cultured for 30 min in the PEMFs with the intension of electromagnetic fields 8 Gs,frequency 15 Hz.MTT assay,alkaline phosphatatase-specific activity assay and tetracycline fluorescence labeling assay were used to observe the effects of PEMFs on the osteoblasts' proliferation,differentiation and bone tissue-like formation.RESULTS: ① MTT assay demonstrated that the A 490 nm value of sham group and PEMFs group was respectively (0.330±0.020) and (0.379±0.038),with obvious difference(P<0.01).② ALP assay displayed that the A 410 nm value of sham group and PEMFs group was respectively (0.170±0.029)and (0.206±0.018),with obvious difference(P<0.01).③ The calcium node in PEMFs group was far more than that in sham group.CONCLUSION: The PEMFs can accelerate the cell proliferation,upregulate the ALP level and increase the bone tissue-like formation of rat osteoblasts in vitro.

关 键 词：脉冲电磁场 成骨细胞 细胞分裂 细胞分化 骨生成 

分 类 号：Q64[生物学—生物物理学]