大鼠血浆中氯解磷定(PAM-Cl)高效液相色谱测定方法的研究  

Determination of pralidoxime chloride in rat plasma by high performance liquid chromatography

作　　者：付朝晖[1] 黄雪祥[1] 肖宏瑞[1] 黄金祥[1] 

机构地区：[1]中国疾病控制中心职业卫生与中毒控制所,北京100050

出　　处：《卫生研究》2005年第2期255-256,共2页Journal of Hygiene Research

基　　金：科技部"十五"科技攻关项目 (No .2 0 0 1BA70 4B0 6)

摘　　要：为给临床上治疗有机磷中毒提供参考依据 ,建立了高效液相色谱法测定大鼠血浆中氯解磷定含量的方法。本方法固定相采用BECKMANC18色谱柱 (ODSdp5u 4 . 6mm× 2. 5cm) ,流动相为乙腈 :(0 . 0 2mol LNaH2 PO4 ,加入 0 .2 %的 1 辛烷基磺酸钠 ,磷酸调节pH至 3 0 ) (体积比 7. 5∶92 .5 ) ,996PDA检测器 ,检测波长为 2 96nm或 2 5 4nm ,保留时间定性 ,峰面积定量。氯解磷定测定的线性范围 1 .0～ 5 0 μg ml,检测限为 0 . 5 μg ml(S N =2 ) ,该方法测得的氯解磷定的回收率为 76 %～ 84 % ,批内 ,批间测定RSD1. 35 % ,2 . 73%Objective\ To provide a reference for clinically curi ng organophosphrous compounds Poisoning. A high performance liquid chromatograph y method has been developed f or determination of pralidoxime chloride in rat plasma. BECKMAN ODS C 18 co lumn, Waters Model 510 HPLC pump and 996 photodiode Detector were used. The mobi le phase consisted of 7.5% acetonitrite and 92.5% (20nmol/L NaH 2PO 4, 0.2% C 8H 17SO 3 Na, pH3.0, adjusted by H 3PO 4 Solution) the flow rate wa s 1.0mol/min. detection wavelength was set at 296. The samples were pretreated with acetonitrite. The results show a good liner correlation between pralidoxime chloride concentration(from 1.0～5.0μg/ml) and absorption intensity. The det ection limit is 0.5μg/ml with signal to noise ratio of 2. The intra-assay and inter-assay coefficients of variation were 1.35% and 2.73%. The recoveries f or plasma were in ranges of 76%～84%.

关 键 词：氯解磷定 高效液相色谱 血浆 

分 类 号：R446.11[医药卫生—诊断学] R595.4[医药卫生—临床医学]