宠物鸟H3N8亚型流行性感冒病毒NA基因分子特征性分析  

Molecular Characteristics of NA Gene of Caged Pet Birds H3N8 Influenza A Virus

作　　者：张云[1] 刘明[1] 于康震[2] Robert G.Webster 

机构地区：[1]St.Jude Children’s Research Hospital [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点开放实验室,哈尔滨150001

出　　处：《病毒学报》2005年第2期145-149,共5页Chinese Journal of Virology

基　　金：National Institute of Allergy and Infectious Diseases Public Health Service Research Grants.Al95357

摘　　要：虽然流行性感冒(流感)病毒的自然宿主是野生水禽类,作为宠物的雀形目鸟(Passeriformes)不代表流感病毒主要储存库[1-4,6,7],但Stallknecht和Shane 1988年对21 318份鸟的样品分析后发现,雀形目鸟中流感病毒的分离率占2.9%,表明雀形目鸟在流感病毒储存库中起着一定的作用.This study characterized the NA genes of five H3N8 caged pet bird influenza viruses from Hong Kong, Indonesia, New Jersey, Singapore and Belgium, respectively. Phylogenetic analysis showed that NA genes of H3N8 caged pet birds influenza viruses are all grouped together and highly related,with 95.9% -99.6% similarity to each other. Especially, the caged pet bird viruses from Hong Kong are more closely related, with 99.6% identity to each other. NA genes of caged pet bird viruses belong to North America lineage. The NA genes of caged pet bird viruses are highly related to A/Mallard/Edmonton/220/90(H3N8) ,with 95.9% -94.2% similarity. The homologies to the Euro-Asian lineage virus A/Duck/Hokkaido/8/80 (H3N8) are only 75.7% -76.4%. Amino acid analysis showed that all the N8 genes of North America influenza viruses, including the caged pet bird viruses in this study,have potential glycosylation(NGS)at 43 - 45 sites,while equine and Euro-A-sia avian influenza viruses have no potential glycosylation at these sites.The potential glycosylation at site 47 might correlate with host species.In general,the N8 genes of caged pet bird H3N8 viruses are molecular closely related to each other and to earlier isolates of H3N8 influenza viruses from different regions of the world indicating that NA genes of these viruses are highly genetically conserved and that a stable lineage occurs in passerine birds. All the NA genes of caged pet bird H3N8 influenza viruses came from a common precursor. Passeriformes plays an important role in transmission and maintenance.

关 键 词：流感病毒 亚型 流行性感冒病毒 分子特征 分离率 自然宿主 宠物 NA基因 野生水禽 禽类 

分 类 号：Q959[生物学—动物学] S858[农业科学—临床兽医学]