二甲亚砜与第二信使效应剂对深低温保存血小板聚集功能的影响  

EFFECTS OF DIMETHYL SULFOXIDE AND SECOND-MESSENGER EFFECTORS ON PLATELET AGGREGATIVE REACTION AFTER CRYOPRESERVATION

作　　者：孟凡会[1] 孙青刚[2] 刘忠强[1] 孙波[3] 刘竹珍[4] 刘金兰[4] 

机构地区：[1]青岛大学医学院附属医院低温医学科,山东青岛266003 [2]青岛大学医学院附属医院,研究生山东青岛266003 [3]青岛大学医学院附属医院输血科,山东青岛266003 [4]青岛大学医学院附属医院血液科,山东青岛266003

出　　处：《齐鲁医学杂志》2005年第1期21-24,共4页Medical Journal of Qilu

基　　金：山东省卫生厅科研基金资助项目(1998CA2CKA1)

摘　　要：①目的观察体积分数0.02二甲亚砜(DMSO)联合第二信使效应剂(ThromboSol)对深低温保存浓缩血小板聚集功能影响.②方法分别用体积分数0.02 DMSO、体积分数0.06 DMSO、体积分数0.02 DMSO+ThromboSol作为保存液,在-196 ℃冷冻保存浓缩血小板,并在冻存后1、3及6个月时分别取出复温,检测各组的血小板计数、二磷酸腺苷(ADP)和花生四烯酸(AA)诱导的聚集反应.③结果冷冻1、3及6个月体积分数0.02 DMSO+ThromboSol组血小板计数、ADP及AA诱导的聚集反应结果与体积分数0.06 DMSO组相比差异无显著意义(F=3.67～5.22,q=0.87～1.89,P>0.05),均高于体积分数0.02 DMSO组,差异有显著意义(q=3.43～4.26,P<0.05).但与新鲜组相比,3组冷冻后各指标结果均有所下降,差异有显著性(q=3.16～4.34,P<0.05).④结论在深低温保存浓缩血小板过程中第二信使效应剂的应用降低了DMSO的使用浓度.Objective To observe the effect of cryopreservation on platelet aggregative reaction by combination use of 0.02 dimethyl sulfoxide(DMSO) plus a mixture of second-messenger effectors(ThromboSol). Methods Platelet concentrates were treated with 0.02 DMSO, 0.06 DMSO and 0.02 DMSO plus ThromboSol, respectively, and then placed in liquid nitrogen. Having been frozen for one, three and six months, the samples were thawed and analyzed. The platelet counts, ADP and arachidonic acid(AA)-induced aggregative reaction were detected. Results The platelet counts of the 0.02 DMSO-plus-ThromboSol group were similar to those of the 0.06 DMSO group(F=3.67-5.22,q=0.87-1.89,P>0.05), while those of the two groups were higher than those of the 0.02 DMSO group (q=3.43-4.26,P<0.05). In comparison to the fresh platelets, the number and functional activity of the frozen platelets of these three groups declined significantly (F=3.67-5.22, q=3.16-4.34,P< 0.05). Conclusion The second-messenger effectors could reduce the DMSO concentration used in cryopreservation.

关 键 词：血小板聚集 二甲亚砜 低温保存 第二信使效应剂 

分 类 号：R331.143[医药卫生—人体生理学]