二甲亚砜与第二信使效应剂对深低温保存血小板超微结构的影响  

EFFECTS OF DIMETHYL SULFOXIDE AND SECOND-MESSENGER EFFECTOR ON CRYOPRESERVATION OF PLATELET ULTRASTRUCTURE

作　　者：孟凡会[1] 孙青刚[2] 王涛[3] 刘竹珍[4] 刘金兰[4] 

机构地区：[1]青岛大学医学院附属医院低温医学科,山东青岛266003 [2]青岛大学医学院,研究生山东青岛266003 [3]青岛大学医学院附属松山医院,山东青岛266003 [4]青岛大学医学院附属医院血液科,山东青岛266003

出　　处：《齐鲁医学杂志》2005年第1期28-30,共3页Medical Journal of Qilu

基　　金：山东省卫生厅科研基金资助项目(1998CA2CKA1)

摘　　要：①目的　观察体积分数0.02二甲亚砜(DMSO)联合第二信使效应剂(ThromboSol)对深低温保存浓缩血小板超微结构的影响。②方法　分别应用3种不同的保存液(体积分数0.02 DMSO、体积分数0.06 DMSO、体积分数0.02 DMSO+ThromboSol)-196 ℃冷冻保存浓缩血小板,并在冻存后1、6个月时分别取出复温,采用扫描和透射电镜观察血小板超微结构变化。③结果　体积分数0.02 DMSO+ThromboSol组血小板能保持完整结构,部分血小板出现激活迹象,冷冻保存1个月和6个月的血小板超微结构无明显差异。④结论　体积分数0.02DMSO+ThromboSol能够有效地保护血小板超微结构,其6个月内的保存效果稳定。Objective To observe the effect of cryopreservation with 0.02 dimethyl sulfoxide(DMSO) and ThromboSol, a mixture of second-messenger effectors, on platelet ultrastructure. Methods The inspissated platelets were treated with 0.02 DMSO, 0.06 DMSO and 0.02 DMSO plus ThromboSol, respectively, and then placed in liquid nitrogen. The samples were thawed and the ultrastructure was observed by SEM and TEM one month and six months later. Results The platelets cryopreserved by 0.02 DMSO and ThromboSol retained good ultrastructure and that some platelets were in active status. There was no significant difference between one-month group and six-month group. Conclusion 0.02 DMSO and ThromboSol might effectively protect the platelet ultrastructure and the long-term cryopreservative effect is stable.

关 键 词：血小板 二甲亚砜 低温保存 第二信使效应剂 超微结构 

分 类 号：R331.143[医药卫生—人体生理学]