钌(Ⅱ)多吡啶配合物与DNA相互作用的等温滴定量热研究(英文)  

Studies on Interaction of Ruthenium Polypyridyl Complex with DNA Using Isothermal Titration Calorimetry

作　　者：徐宏[1,2,3] 张鹏[1] 杜芬[1] 周兵瑞[1] 梁毅[1] 刘剑洪[4] 刘志刚[4] 计亮年[5] 

机构地区：[1]武汉大学生命科学学院病毒学国家重点实验室 [2]深圳大学师范学院化生教育系,深圳518060 [3]南京大学配位化学国家重点实验室,南京210093 [4]深圳大学师范学院化生教育系 [5]中山大学化学与化工学院

出　　处：《无机化学学报》2005年第4期468-471,共4页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.30370309,90408012);国家 863 计划(No.2004AA404260);中国博士后科学基金(No.2003034503);南京大学配位化学国家重点实验室开放课题资助项目。

摘　　要：The interaction of ruthenium polypyridyl complex with DNA has been studied by isothermal titration calorimetry (ITC). The results show that complex [Ru(phen)2PMIP]2+ {phen=1,10-phenanthroline, PMIP=2-(4-methylphenyl)imidazo1,10-phenanthroline} interacts with calf thymus DNA (CT DNA) in terms of a model for a single set of identical sites through intercalation. The results are in agreement with our previous observations from spectroscopic methods and viscosity measurements. In addition, the results further show that the driving force for DNA binding with the complex is mainly driven by the enthalpy changes, and the contribution from the entropy changes to this driving force is negligible.The interaction of ruthenium polypyridyl complex with DNA has been studied by isothermal titration calorimetry (ITC). The results show that complex [Ru(phen)2PMIP]2+ {phen=1,10-phenanthroline, PMIP=2-(4-methylphenyl)imidazo1,10-phenanthroline} interacts with calf thymus DNA (CT DNA) in terms of a model for a single set of identical sites through intercalation. The results are in agreement with our previous observations from spectroscopic methods and viscosity measurements. In addition, the results further show that the driving force for DNA binding with the complex is mainly driven by the enthalpy changes, and the contribution from the entropy changes to this driving force is negligible.

关 键 词：钌(Ⅱ)多吡啶配合物 DNA 相互作用 等温滴定量 

分 类 号：O614.821[理学—无机化学] O641.4[理学—化学]