铬(Ⅵ)与谷胱甘肽作用及其中间态配合物形成的电化学表征  

Electrochemical Characterization on the Formation of Chromium(Ⅵ) and Glutathione Intermediates

作　　者：杨培慧[1,2] 张文豪[1] 赵秋香[1] 蔡继业[1] 

机构地区：[1]暨南大学化学系 [2]中国科学院安徽光学精密机械研究所,合肥230026

出　　处：《无机化学学报》2005年第4期495-499,共5页Chinese Journal of Inorganic Chemistry

基　　金：广东省自然科学基金(No.021190);广州市科技项目(No.2003Z3-D2041)资助。

摘　　要：本文采用电化学方法,对谷胱甘肽(GSH)与重铬酸钾的相互作用及其中间态配合物的形成过程进行研究。结果表明:在pH=5.6的HAc鄄NaAc缓冲溶液中,GSH浓度为Cr髩浓度5倍以上时,Cr髩与GSH作用完全并形成一新的中间态配合物,该中间态配合物于+0.21V和+0.36V(vsSCE)处产生一对新的氧化还原峰,UV鄄Vis的吸收光谱进一步证明了中间态配合物的形成。该配合物不稳定,在一定时间内缓慢分解,其电化学与UV鄄Vis光谱动力学信息同步。进一步探讨了GSH与Cr髩作用的电极反应机理。当Zn髤存在于该体系时,Zn髤对中间态配合物的生成和分解过程起着双向催化作用。The interaction between chromium?髩 and Glutathione (GSH) and the formation of their intermediate complexes were studied by electrochemical method. The results showed that a new pair of redox peaks were obtained at +0.21 V and +0.36 V (vs SCE) in acetate buffer solution (pH 5.6) when the concentration of GSH was five times more than that of Cr?髩. The electrochemical dynamics revealed that the peaks of +0.21 V and +0.36 V were the redox peaks of the intermediate complex, the conclusion was further confirmed by UV-Vis absorption spcetra. The intermediate complex was unstable and decomposed gradually in a certain period of time. When zinc ion was added to the above solution, both the current of redox peaks and the intensity of the UV-Vis absorbance were enhanced. Therefore, the zinc ion could catalyze the formation and decomposition of the Cr?髩-GSH intermediate complex. The mechanism of the electrode reaction was also discussed.

关 键 词：重铬酸钾 谷胱甘肽 电化学分析 中间态配合物 双向催化作用 浓度 

分 类 号：O614.611[理学—无机化学] O641.4[理学—化学]