早期自然流产患者TNF-α及TGF-β1的异常表达  

Abnormal Expression of TNF-α and TGF-β_1 mRNA in Spontaneous Abortion

作　　者：包洪初[1] 胡继芬[2] 吕娜[1] 

机构地区：[1]山东烟台毓璜顶医院生殖医学中心,烟台264000 [2]福建医科大学附属第一医院妇产科,福州350005

出　　处：《生殖与避孕》2005年第3期149-154,共6页Reproduction and Contraception

摘　　要：目的:探讨自然流产患者外周血单个核细胞和胎盘组织肿瘤坏死因子-α(TNF-α)以及转化生长因子-β(TGF-β1)的异常表达。方法:采用RT-PCR技术,检测30例早期自然流产患者(自1然流产组)、25例正常妊娠妇女(正常妊娠组)、25例正常未妊娠妇女(未孕对照组)外周血单个核细胞内TNF-αmRNA和TGF-β1mRNA表达水平;采用免疫组织化学技术检测绒毛和蜕膜组织内TNF-α及TGF-β1表达强度。结果:三组研究对象外周血单个核细胞表达TNF-αmRNA相对含量分别为21.1±9.4%、68.6±14.1%、97.0±11.6%,组间比较有显著性差异(P<0.05);TGF-β1mRNA相对含量分别为21.5±9.4%、95.7±12.9%、87.0±13.8%,组间比较也均有显著性差异(P<0.05)。自然流产组细胞滋养层细胞及合体滋养层细胞表达TNF-a均显著强于人工流产组(P<0.01);自然流产组合体滋养层细胞表达TGF-β1显著低于人工流产组(P<0.01)。结论:①早期自然流产患者外周血单个核细胞TNF-αmRNA表达水平显著下降,而细胞滋养层细胞及合体滋养层细胞表达TNF-α均显著强于人工流产组,提示TNF-α可能在母胎界面局部发挥作用并导致流产。②正常早期妊娠妇女外周血单个核细胞TGF-β1mRNA表达水平较正常未妊娠妇女显著升高,提示TGF-β1对妊娠维护起重要作用。早期自然流产患者外周血单个?Objective: To investigate the abnormal expressions of TNF-α and TGF-β1 in peripheral blood mononuclear cells (PBMCs) and placental tissues in patients with early spontaneous abortion.Methods: Using the technique of semi-quantitative reverse transcript-polymerase chain reaction (RT-PCR), TNF-α mRNA and TGF-β1 mRNA in PBMCs were measured in spontaneous abortion group (30 cases), normal pregnancy group (25 cases) and non-pregnant group (25 cases). The expressive intension of TNF-α protein and TGF-β1 protein in placental tissues was also identified by immunohistochemistry. Results: Both levels of TNF-α mRNA and TGF-β1 mRNA expressed in PBMCs were significantly different between the three groups respectively (P<0.05). Levels of TNF-α in syncytiotrophoblastic and cytotrophoblastic cells of the two aborted groups were substantially higher than the non- pregnant group (P<0.01), but the levels of TGF-β1 in syncytiotrophoblastic cells of the two aborted groups were markedly lower than the non-pregnant group (P<0.01). Conclusion: There is potential relation between TGF-β1 at the fetomaternal interface and spontaneous abortion. TGF-β1 may contribute to the maintenance of pregnancy, and low-level expression of TGF-β1 may be associated with pregnancy failure.

关 键 词：自然流产 肿瘤坏死因子 转化生长因子Β 聚合酶链反应 免疫组织化学 

分 类 号：R714.21[医药卫生—妇产科学]