微弧氧化和电泳沉积复合制备羟基磷灰石/TiO_2复合涂层及其生物学特性  

HYDROXYAPATITE/TiO_(2) COMPOSITE COATINGS BY MICRO-ARC OXIDATION AND ELECTROPHORESIS DEPOSITION AND ITS BIOLOGICAL PROPERTIES

作　　者：马楚凡[1] 李冬梅[2] 李贺军[1] 蒋百灵[3] 李佐臣[4] 王立新[4] 

机构地区：[1]西北工业大学材料科学与工程学院,西安710072 [2]第四军医大学口腔医学院修复科,西安710032 [3]西安理工大学材料学院,西安710048 [4]西北有色金属研究院生物工程材料研究中心,西安710016

出　　处：《硅酸盐学报》2005年第3期323-329,共7页Journal of The Chinese Ceramic Society

基　　金："863"计划(2002AADF3304);国家科技攻关计划(2002BA330C)资助项目。

摘　　要：采用微弧氧化和电泳沉积复合的方法,在钛样品表面沉积了羟基磷灰石(hydroxyapatite,HA)/TiO2复合涂层。用扫描电子显微镜, 能量散射X射线能谱分析和X射线衍射对复合涂层的表面形貌、形成过程、元素组成及含量变化、相组成进行了观察和分析。通过剪切粘结 实验确定了复合涂层与基体的结合强度,并在高温消毒后,将成骨样细胞MC3T3E1接种于试件表面进行培养以评估HA/TiO2复合涂层 的生物学性能。结果表明:形成的复合涂层由内层致密的TiO2层、中间HA/TiO2混合过渡层和外层HA层构成,其中HA含量呈梯度变化, 使TiO2层逐渐向HA层过渡。HA/TiO2复合涂层和基体间的结合强度约为(19.3±2.5)MPa。通过细胞免疫荧光染色观察到细胞在培养 3h就可附着于复合涂层表面并形成良好的铺展,而且随着培养时间的延长,细胞的形态更加舒展,附着在复合涂层表面的细胞数量亦逐渐增 加,初步表明复合涂层具有较好的生物学和力学性能。A layered HA(hydroxyapatite)/TiO2 composite coaling was deposited on pure titanium using micro-arc oxidation pre-treatment and electrophoresis deposition technique. Surface morphology and formation procedure of the coating at various stages were observed by scanning electron microscope. Element component and phase component of the coating was analyzed by energy dispersive X-ray analysis and X-ray diffraction. The adhesive strength of the coating was tested by shear bonding experiment. In order to investigate the bioactivity and biocompatibility of the coating, the titanium discs with the HA/TiO2 coating were sterilized and after that, MC-3T3-El osteoblast-like cells were cultured on the surface of the discs. The results indicate that the coating consists of the dense TiO2 interlayer, the intermediate layer of a composite of the HA/TiO2 coating and the top pure HA layer. The average adhesive strength of the coating is (19.3+2.5) MPa. The observation of immunofluorescence coloration indicates that osteoblast-like cells can build good adhesion to the coating and spread well after cultured for 3 h. The amount of cells attached to the coating increases with the increase of time. So it shows that the HA/TiO2 coating deposited by micro-arc oxidation and electrophoresis deposition technique has good bioactivity and biocompatibility, and it possesses a very good combination of biochemical stability and mechanical properties.

关 键 词：微弧氧化 电泳 羟基磷灰石/二氧化钛 复合涂层 钛种植体 生物学评价 

分 类 号：O484[理学—固体物理]