TGF-β1对醛糖还原酶在大鼠系膜细胞及Thy-1肾炎中表达的影响  

Effect of transforming growth factor-β1 on aldose reductase expression in rat mesangial cells and anti-Thy1 glomerulonephritis

作　　者：蒋涛[1] 杨静[1] 李慧[1] 赵仲华[1] 张秀荣[1] 张农[1] 

机构地区：[1]复旦大学上海医学院病理系,上海200032

出　　处：《临床与实验病理学杂志》2005年第1期90-94,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金资助(30170430)

摘　　要：目的　研究转化生长因子β1(TGF- β1)对醛糖还原酶(AR)在大鼠系膜细胞(MsC)及大鼠抗Thy 1肾小球肾炎模型 (ATG)中表达的影响。方法　采用RT PCR和Westernblot技术分别检测TGF β1刺激后体外培养大鼠系膜细胞AR的表达以 及TGF -β1和AR在ATG中的表达;应用免疫组化检测ATG中TGF -β1、AR的表达,并对染色强度进行图像分析。结果　外源 性TGF- β1作用后,大鼠系膜细胞中AR表达升高,并显示出一定的时间与剂量依赖性。ATG中,随着病程的延长,TGF -β1、AR 表达升高,对免疫组化结果进行图像分析显示,二者表达之间具有相关性(r=0.65,P<0.05)。结论　TGF -β1可以上调其相 关反应性基因AR的表达,后者可能参与了肾小球肾炎发生发展的过程。Purpose To study the influence of transforming growth factor β1 (TGF-β1) on the expression of aldose reductase (AR) in rat mesangial cells and anti-Thy1 glomerulonephritis. Methods RT-PCR,Western blot and immunohistochemistry were used to detect the AR expression in rat mesangial cells and anti-Thy1 glomerulonephritis. Image analysis software was used to analyze the staining intensity of TGF-β1 and AR. Results Elevated levels of AR mRNA and protein were found in rat mesangial cells with stimulation of TGF-β1,and the elevation was in a time and dose-dependent manner . In rat anti-Thy1 glomerulonephritis elevated level of TGF-β1 was positively correlated with that of AR. Conclusions TGF-β1 up-regulates the expression of AR,which may be involved in the pathogenesis of glomerulonephritis.

关 键 词：TGF—β1 表达 系膜细胞 AR 大鼠 醛糖还原酶 肾炎 RT—PCR 转化生长因子βl 体外培养 

分 类 号：R692[医药卫生—泌尿科学] R285.5[医药卫生—外科学]