圣诞树和黑荆树根瘤菌的分离及回接  

Isolation and Backward Inoculation of Nodule Bacteria for Tree Species of Acacia dealbata and Acacia mearrnsii

作　　者：贾风勤[1] 杨比伦[1] 

机构地区：[1]西南林学院资源学院,云南昆明650224

出　　处：《西南林学院学报》2004年第4期9-11,共3页Journal of Southwest Forestry College

基　　金：世界自然基金会(WWF)资助项目.

摘　　要：从圣诞树(AcaciadealbataLinn)、黑荆树(AcaciamearrnsiiDeWilld)的根瘤中分离培养各获得3个单菌落.初步鉴定为根瘤菌(Rhizobium.spp).挑选圣诞树03-A-1菌株、黑荆树03-D-2菌株分别回接于用灭菌无氮培养液培养的圣诞树、黑荆树幼苗,并与对照组对照,回接植株有根瘤形成.表明03-A-1,03-D-2菌株是根瘤菌菌株.Two leguminous tree species i.e.,Acacia dealbata and Acacia mearrnsii were observed in semi-sealed tube with sterilized non-nitrogen solution, and nodule bacteria were isolated from root nodules of both these two species by following the method suggested by Vincent(1970). The preliminary determination indicated that the nodule bacteria were Rhizobium spp.. Backward inoculation of bacteria colony 03-A-1 from Acacia dealbata was done to yang seedlings of the same tree species and root nodules were obtained, and bacteria colony 03-D-2 from Acacia mearrnsii was backward inoculated to seedlings of the same tree species and the same result was obtained. It was indicated that the colonies of 03-A-1 and 03-D-2 should be the nodule bacteria of these tree species.

关 键 词：根瘤菌 树根 菌株 植株 幼苗 挑选 培养液 对照组 菌落 分离 

分 类 号：S565[农业科学—作物学]