泥蚶体液凝集素性能的初步研究  

Preliminary Study On The Humoral Hemagglutinin Properties of Tegillarca granosa Linnaeus

作　　者：柯佳颖[1] 陈寅山[1] 黄素华[2] 

机构地区：[1]福建师范大学生物工程学院,福建福州350007 [2]福州市第二医院输血科,福建福州350007

出　　处：《漳州师范学院学报（自然科学版）》2005年第1期65-68,112,共5页Journal of ZhangZhou Teachers College（Natural Science)

基　　金：福建省教育厅资助项目(JA02179)

摘　　要：在泥蚶(Tegillarca granosa Linnaeus)体液中发现有凝集素存在.利用14种天然或经酶处理的动物和人红细胞来测定泥蚶体液凝集素的凝集活力,并且进行糖(200 mmol·L-1)抑制、热稳定性、pH及Ca2+影响试验.结果显示:泥蚶体液凝集素对14种红细胞均有凝集作用.其中对兔红细胞的凝集作用可被乳糖和半乳糖所抑制,凝集活性依赖于Ca2+,在pH7.0较稳定,温度为40～60℃范围内均具较高活性,经90℃保温10 min后活性完全消失.The hemagglutinin was found in the humor of Tegillarca granosa Linnaeus. The hemagglutinin was detected for hemagglutination with 14 natural and enzyme-treated erythrocytes of human and animals, in addition, glyco-inhibiting action(200mmol·L-1), thermostability and influrence of pH and Ca2+ on the activity of the sample were also tested. The results showed the humoral hemagglutinin could agglutinate 14 kinds of erythrocytes, and the agglutination activity to rabbit erythrocytes was inhibited by lactose and D-galactose. The hemagglutination activity of humor depended on Ca2+, and it showed the highest hemagglutination activity in pH 7.0, temperature range of 40℃~60℃, the agglutinating activities was completely destroyed after heated at 90℃ for 10min.

关 键 词：体液 凝集素 CA^2+ 初步研究 人红细胞 抑制 半乳糖 泥蚶 影响试验 动物 

分 类 号：S968[农业科学—水产养殖] R446[农业科学—水产科学]