信号转导子与转录激活子3蛋白在金黄地鼠视网膜生后早期发育过程中的表达  

EXPRESSION OF STAT3 PROTEIN IN THE RETINA OF HAMSTER DURING EARLY POSTNATAL DEVELOPMENT

作　　者：张艳[1] 于恩华[1] 刘猛[1] 

机构地区：[1]北京大学医学部解剖学系,北京100083

出　　处：《解剖学报》2005年第1期10-13,共4页Acta Anatomica Sinica

基　　金：北京大学"985"青年科研启动基金 ;北京市自然科学基金资助项目 (70 2 2 0 18)

摘　　要：目的　观察信号转导子与转录激活子 3(STAT3)蛋白在金黄地鼠视网膜生后早期发育过程中的表达。　方法　STAT3蛋白免疫细胞化学染色和Western免疫印迹方法　结果　生后发育早期整个视网膜神经层均有STAT3阳性产物分布 ,以视网膜节细胞层 (GCL)、内网层 (IPL)和成神经细胞层 (NBL)的内层更明显 ,随着发育进程 ,其阳性产物逐渐局限于视网膜节细胞的胞浆和胞核 ;蛋白半定量结果显示 ,生后 1周内STAT3蛋白表达水平较高 ,之后逐渐降低 ,成年后最低。　结论　STAT3蛋白的表达及变化可能与视网膜生后早期发育密切相关。Objective To investigate the expression and distribution of STAT3 in the retina during the early development of postnatal hamster. Methods Immunocytochemical method and Western blot analysis were used. Results The expression of STAT3 was found in all layers of the retina in all newborn hamsters,most pronounced in the ganglion cell layer(GCL),the inner plexiform layer(IPL)and the inner area of neuroblastic layer(NBL).With the development,the STAT3 immunoreactivity was gradually restricted in the cytoplasm and the nucleus of the retinal ganglion cells.The result of the Western blot showed that the STAT3 was highly expressed in the first week of postnatal development and then reduced gradually till the lowest point in the adulthood.Conclusion The expression of STAT3 protein may be closely related to the early development of the retina in the postnatal hamster.

关 键 词：STAT3蛋白 视网膜 生后发育 免疫细胞化学 免疫印迹法 金黄地鼠 

分 类 号：R322.91[医药卫生—人体解剖和组织胚胎学]