脂多糖对大鼠中脑和桥脑内Fos、GFAP、OX42表达的影响  

THE EFFECT OF LIPOPOLYSACCHARIDE ON THE EXPRESSION OF FOS,GFAP AND OX42 IN RAT MIDBRAIN AND PONS

作　　者：兰莉[1] 高蓓[1] 饶志仁[1] 

机构地区：[1]第四军医大学神经科学研究所,西安710032

出　　处：《解剖学报》2005年第1期24-27,共4页Acta Anatomica Sinica

基　　金：军队"十五"重点课题资助项目 (0 1Z0 82 )

摘　　要：目的　探讨单次腹腔注射脂多糖 (LPS) ,中脑和桥脑神经元、星形胶质细胞和小胶质细胞的可塑性变化。方法　抗Fos蛋白、抗胶质原纤维酸性蛋白 (GFAP)、抗特异性标记小胶质细胞 (OX4 2 )和抗Fos/GFAP双重免疫组织化学标记法。结果　Fos阳性神经元分布于上丘、中脑导水管周围灰质、臂旁核和蓝斑。Fos蛋白在注射后 30min表达 ,1～ 3h为高峰。GFAP阳性星形胶质细胞胞体变大 ,突起增粗 ,细胞密度增加 ,30min出现表达 ,1h为高峰 ,3h后减少。 0X4 2阳性小胶质细胞首先于脑室周围灰质表达 ,注射后 6h达到高峰 ,胞体变大 ,全脑分布。相应于Fos阳性神经元分布区域 ,GFAP阳性星形胶质细胞和OX4 2阳性小胶质细胞深染和密集。结论　上丘、臂旁核、蓝斑内神经元、星形胶质细胞、小胶质细胞可能参与神经免疫调节 ,臂旁核可能为此调节通路的中继站之一。Objective To investigate the plastic changes of neurons,astrocytes and microglials in rat midbrain and pons after a single intraperineal(IP)injection of lipopolysaccharide(LPS). Methods Anti-Fos,anti-GFAP,anti-OX42 single-labeling and Fos/GFAP double-labeling immunohistochemical ABC methods were used in this study. Results Fos(+)neurons were distributed in the superior colliculus,periaqueductal gray,parabrachial nuclei,locus ceruleus.The Fos(+) neurons appeared at 30min,and peaked at 1-3h after injection.The number of GFAP(+)astrocytes increased at 30 min,peaked at 1h,decreased at 3 h.Their bodies hypertrophied and processes thicken.OX42(+)microglials appeared firstly in periventricular gray at 30min after injection,peaked 6h.It became bigger and ramified.The distribution of Fos(+)neurons,GFAP(+)astrocytes and OX42(+)microglials were the same.Conclusion The neurons,astrocytes and microglials in superior colliculus,parabrachial nuclei and locus ceruleus might be involved in the neuroimmunomodulation of central nervous system to peripheral-immunity stimulate.The parabrachial nuclei may be a relay station of neuroimmunomodulation route.

关 键 词：脂多糖 神经元 小胶质细胞 星形胶质细胞 神经免疫调节 免疫组织化学 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]