除草剂毒莠定原药的致突变性  

Mutagenicity of Picloram in Herbicide

作　　者：肖芸[1] 陈琼姜[1] 顾刘金[1] 于仲波[1] 陈秀风[1] 吴立仁[1] 吴南翔[1] 张幸[1] 

机构地区：[1]浙江省医学科学院卫生学研究所,杭州市310013

出　　处：《职业与健康》2005年第4期491-493,共3页Occupation and Health

摘　　要：目的　研究除草剂毒莠定原药的致突变性。方法　采用小鼠骨髓多染红细胞微核试验,剂量设为雄性13 1、2 61、5 2 2mg/kg、雌性2 3 0、460、92 0mg/kg ;小鼠睾丸精母细胞染色体畸变试验,剂量设为3 2 6、65 3、13 0 5mg/kg。Ames试验用TA97、TA98、TA10 0、TA10 2菌株,加与不加S9试验,剂量设为5、5 0、5 0 0、10 0 0、5 0 0 0 μg/皿。结果　小鼠骨髓多染红细胞微核试验和小鼠睾丸精母细胞染色体畸变试验,各剂量组和阴性对照组比较,差异无显著性(P >0 0 5 ) ;Ames试验中各测试浓度的回变菌落数均末超过自然回变菌落数的2倍。结论　该试验范围内,未见毒莠定原药有致突变性。[Objective] To study the mutagenicity of Picloram in herbicide. [Methods] Medulla polychromatic erythrocyte micronucleus test was carried out with doses of 131, 261 and 522 mg/kg respectively on male mice and 230, 460 and 920 mg/kg respectively on female mice; testicle spermatocyte chromosome aberrance test was carried out with doses of 326, 653 and 1 305 mg/kg respetively; TA97, TA98, TA100 and TA102 strains were used in the Ames test (with or without S9 test) with doses of 5, 50, 500, 1 000 and 5 000 ug/皿 respectively. [Results] No significant difference was found in the comparison between different dose groups with the negative group in the medulla polychromatic erythrocyte micronucleus test and testicle spermatocyte chromosome aberrance test (P>0.05); recovered strain numbers in different concentrations in the Ames test were not more than twice of the natural recovered strain numbers. [Conclusion] No mutagenicity was found in Pocloram within the range of this test.

关 键 词：毒莠定 微核试验 精母细胞染色体畸变试验 AMES试验 致突变性 

分 类 号：R114[医药卫生—卫生毒理学]