HPLC测定中成药大活络丸（古方）中微量麻黄碱的含量  

HPLC Determination of Trace Ephedrine in Loose-Jointed Pill of Chinese Traditional Medicine

作　　者：胡家炽[1] 何志强[1] 黄钦鸣 

机构地区：[1]广州市药品检验所

出　　处：《药物分析杂志》1994年第5期33-36,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：本文采用ＨＰＬＣ法，用茴香醛作内标，测定中成药大活络丸中微量麻黄碱，方法采用蒸馏接收麻黄碱并定量氧化生成苯甲醛，因而具有高的检测灵敏度和适合多种含麻黄碱制剂的含量测定。在１～５μｇ／ｍｌ的浓度范围具有良好的线性关系，ｒ＝０．９９９５，平均回收率（ｎ＝５）９９．４％，ＲＳＤ＝１．５％。The paper reports the determination method of trace ephedrine in Loose-Jointed pill of Chinese traditional medicine (contains herba ephdrae, cow-bezoar, scorpion, musk etc. ), the ephedrine was collected by steam-distillation and completely oxidized into benzaldehyde determined by HPLC with anisaldehyde as the internal standard. The method has higher sensitivity.The standard curve was linear in the concentration range of 1～5μg/ml (r=0. 9995), the average recovery 99.4% (n=5),RSD=1.5%.

关 键 词：大活络丸 麻黄碱 高效液相色谱 

分 类 号：R286.0[医药卫生—中药学]