反相离子对色谱法测定兔血清中二氯乙酸钠的浓度  

Determination of Sodium Dichloroacetate in Serum of Rabbit by Ion-Pair RP-HPLC

作　　者：文爱东[1] 顾斌[1] 王纪保[1] 宋龄 蒋永培[1] 

机构地区：[1]第四军医大学西京医院药剂科

出　　处：《药物分析杂志》1994年第5期28-31,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：建立了反相离子对色谱法测定兔血清中二氯乙酸钠含量的方法。ＤＣＡ－Ｎａ在碱性条件下加热与水合肼定量生成稳定且有较强吸收系数的腙，以离子对色谱试剂ＩＰＲ－Ｂ_８为对离子，在ＺｏｒｂａｘＯＤＳ（４．６ｍｍ×２５０ｍｍ）柱上，以水－甲醇－ＩＰＲ－Ｂ_８（６４：３４：２，Ｖ／Ｖ）为流动相得到较好的分离，灵敏度０．０４ＡＵＦＳ，在２６５ｎｍ处测定，衍生物线性范围在０．６～５０．０μｇ／ｍｌ，ｒ＝０．９９９４，平均回收率为９８．０±３．６％，日内及日间精密度ＲＳＤ分别为２．６％和３．３％。以本法测定了ＤＣＡ－Ｎａ在兔体内的血药浓度及药代动力学参数。An ion-pair RP-HPLC method was established for the determination of sodium dichloroacetate (DCA- Na) in rabbit serum. The derivative of DCA-Na was produced through reacting DCA-Na with hydrazine hydrate in basic solution (100℃) . The PH was adjusted to 2～3. The mobile phase consisted of a mixture of H_2O-CH_3OH-IPR-B_8 (64 :34 :2 PH=3. 0) . The analytical column was Zorbax ODS (4. 6mm×250mm 5μm) . The derivative of DCA-Na was detected at 265nm and sensitivity of 0. 04 AUFS, recovery was 98. 0± 3.6%.The RSD's of within-day and day-to-day were less than 5.0%. The method has been applied to pharmacokinetics study.

关 键 词：二氯乙酸钠 血药浓度 药代动力学 

分 类 号：R969.1[医药卫生—药理学]