高氟对大鼠口腔粘膜细胞增殖周期和DNA合成的影响  

Effects of excessive fluoride on oral mucosal cell DNA synthesis and cell cycle changes in rats

作　　者：贺凌飞[1] 黄闽[1] 王阅春[1] 陈建刚[1] 

机构地区：[1]武汉大学中南医院口腔科,武汉430071

出　　处：《中国地方病防治》2004年第3期129-131,共3页Chinese Journal of Control of Endemic Diseases

摘　　要：目的　研究高氟对大鼠口腔粘膜细胞增殖周期和DNA合成的影响。方法　雄性SD大鼠饮用含 15 0mg/LNaF的蒸馏水溶液 4周 ,用流式细胞术研究大鼠口腔粘膜细胞增殖周期和DNA相对含量 (DNARC) ,用单细胞凝胶电泳检测DNA损伤。结果　高氟能引起大鼠口腔粘膜细胞增殖周期的改变 ,使G2 /M期细胞明显下降 ,DNARC也显著下降 ,DNA损伤率明显升高。另外 ,高氟也造成口腔粘膜组织的氧化应激 ,活性氧族、脂质过氧化产物显著增高 ,还原型谷胱甘肽明显下降。结论　高氟不仅改变了大鼠口腔粘膜细胞的增殖周期 ,还造成氧化应激 。Objective To explore effects of excessive fluoride on oral mucosal cell DNA synthesis and cell cycle changes in rats. Methods Two groups of male SD rats were provided with deionized drinking water containing 0(control group) and fluoride(150 mg/L sodium fluoride) respectively for 4 weeks. Oral mucosal cell DNA damage was measured with single cell gel electrophoresis(or comet assay), DNA relative content(DNA RC ) and cell cycle were detected with flow cytometry. Results Excessive fluoride could reduce the cell number of G 2/M period in cell cycle and decrease DNA relative content significantly in rat oral mucosa. In addition, excessive fluoride could induce rat oral mucosal oxidative stress. The DNA damage rates of excessive fluoride group were 50.20% in oral mucosal cells and were higher than that of the control group significantly. Conclusions It was suggested that excessive fluoride could induce cell cycle changes, decrease DNA relative content and result in oxidative stress and DNA damage in rat oral mucosal cells in vivo.

关 键 词：口腔粘膜细胞 增殖周期 大鼠 高氟 DNA合成 DNA损伤 氧化应激 相对含量 下降 雄性 

分 类 号：R599.9[医药卫生—内科学] R285.5[医药卫生—临床医学]