高效氯氰菊酯对德国小蠊Na-K-ATP_(ase)抑制效应  

Inhibition of high effect cypermethrin on Na-K-ATP_(ase) of blattella germa nica

作　　者：马义[1] 付荣恕[1] 霍新北[2] 马民[3] 

机构地区：[1]山东师范大学生命科学学院,济南250014 [2]山东省疾病预防控制中心 [3]暨南大学医学院

出　　处：《中国公共卫生》2004年第12期1440-1441,共2页Chinese Journal of Public Health

基　　金：山东省卫生厅科技发展计划项目 (HZ 0 990 )

摘　　要：目的　通过研究高效氯氰菊酯与德国小蠊Na K ATPase活性水平的量效与时效关系 ,进一步完善高效氯氰菊酯作用机制的研究 ,为德国小蠊的防制工作提供理论基础和方法参考。方法　参照国标GB 13917 1～ 13917 8- 92《农药登记卫生杀虫剂室内药效试验方法》喷施一定剂量不同浓度的高效氯氰菊酯 ,用ATP酶测定试剂盒测定德国小蠊Na K ATPase活性。结果　 4 8h内高效氯氰菊酯对德国小蠊Na K ATPase活性的抑制作用呈浓度梯度效应 ,高效氯氰菊酯的浓度与德国小蠊 72h的死亡率和 14 4h内对Na K ATPase活性的抑制时效呈正相关。结论　Na K ATPase是高效氯氰菊酯的作用靶标之一 ,Na K ATPase敏感度降低而产生的靶标抗性可能是德国小蠊对高效氯氰菊酯产生抗药性的重要原因之一。Objective To undestand the operational mec hanisms of high effect cypermethrin and to provide theoretical basis and useful met hods by researching the dosage effect and the time effect between high effect cy permethrin and Na-K-ATP ase activities of Blattella germanica. Methods Quantitative high effect cypermethrin of differen t concentration was sprayed according to the method of GB 13917.1-13917.8—9 2 for insec ticide registered testing indoor effect.The activities of Na-K-ATP ase we re determined by using ATP ase Kit.Results Inhibition of high effect cypermethrin on Na-K- ATP ase activities of Blattella germanica showed concentration gradient effect in 48 hours.The correlation of concentration of high effect cypermethrin and inhibiting time effect on Na-K-ATP ase activities of Blattella germanica in 144 hours and death rate of Blattella germanica in 72 hours was positive relationship.Conclusion Na-K-ATP ase is one of the targets of h igh effect cypermethrin.Because of sensitivity decrease of Na-K-ATP ase,t arget resistance may be one of the important resistant mechanisms to high effect cypermethrin.

关 键 词：德国小蠊 NA-K-ATPASE 高效氯氰菊酯 抗药性 

分 类 号：R979.8[医药卫生—药品]