高效液相色谱法测定氟康唑血药浓度  

High Performance Liquid Chromatographic Determination of Fluconazole in Serum

作　　者：白林[1] 张志萍[1] 陈秀兰[1] 徐向阳[1] 

机构地区：[1]中国人民解放军总医院

出　　处：《药物分析杂志》1994年第2期22-23,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：氟康唑（Ｆｌｕｃｏｎａｚｏｌｅ）为新型三唑类抗真菌药物。本文采用高效液相色谱法测定其血药浓度，ＯＤＳ色谱柱（１５０×４．６ｍｍ，１０μｍ），流动相为乙腈－０．０５ｍｏｌ／Ｌ，磷酸二氢钾溶液（２３∶７７，Ｖ／Ｖ），非那西丁为内标，标准曲线回归方程为Ｙ＝０．２５９５Ｘ－０．０１１９８，ｒ＝０．９９９９，３种浓度平均回收率１０１．５８％。并进行了１０名健康志愿者的血药浓度测定。Fluconazole is a novel triazole antifungal agent. A sensitive HPLC method has been developed for the determination of fluconazole in serum. Phenacetin was used as the intermal standard. Fluconazole analysis was carried out by using ODS column ( 150 × 4. 6mm I D), the mobile phase consisted of a mixture of acetonitrile-0.05mol/L potassium dihydrogen phosphate (23 ∶ 77, V/V). The standard curve regression equation was Y= 0. 2595X-0. 01198 (r= 0. 99998). The average recovery of three concentrations was 101. 58%. The pressent assay procedure has been applied to monitoring serum levels in ten volunteers.

关 键 词：氟康唑 高效液相色谱 药代动力学 

分 类 号：R969.1[医药卫生—药理学]