HPLC柱切换法血浆直接进样测定环丙氟哌酸  

Determination of Ciprofloxacin in plasma by Direct Injection Using HPLC Column Switching Technique

作　　者：李章万[1] 郭平[1] 郭群[1] 

机构地区：[1]华西医科大学药学院

出　　处：《药物分析杂志》1994年第2期16-18,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：本文用ＨＰＬＣ柱切换技术，以ＭｅｒｃｋｌｉｃｈｒｏｐｒｅｐＲＰ２（２５～４０μｍ）为预处理柱填料，ＳｈｉｍｐａｃｋＣＬＣ－ＯＤＳ为分析柱，水为预处理流动相，０．２ｍｏｌ／Ｌ乙酸和乙酸铵为净化清洗液，在线净化富集样品；通过柱自动切换，以甲醇－乙酸铵（０．２ｍｏｌ／Ｌ，ｐＨ２．７）（３２∶６５）为分析流动相，将净化富集于预处理柱上的被测组分冲至分析柱上分离，在２８０ｎｍ处测定。本法操作简单，血浆中环丙氟哌酸含量以外标法定量，日间相对标准偏差为０．７～２．２％，日内相对标准偏差为１．１～３．３％，在浓度为１０３～５１５０ｎｇ／ｍｌ范围内线性良好，ｒ＝０．９９９９，最低检测浓度达５２ｎｇ／Ｍｌ血浆。An automated clean-up and concentration method by column switching is described for the assay of ciprofloxacin, an antimicrobial agent, in plasma. The system uses Merck Lichoprep RP 2 (25～40μm) as a short precolumn packing material for on-line clean-up and concentration. Shimpack CLC-ODS(5μm) is used as analytical colunm. Water is used as the pretreatment mobile phase and a mixture of methanol-0.2mol/L ammonium acetato solution (pH2. 7) (32 ∶ 65) is used as the analytical mobile phase. The plasma is diluted with a solution of 0.2mol/L acetic acid and injected directly on the precolunm. A solution of 0.2mol/L acetic acid and a solution of 0. 2mol/L acetic acid-ammonium acetate are used as the purge solution for clean-up. The detection is at 280 nm and no internal standard is needed.The RSD within day are 1. 1～3. 3% and the RSD between days are 0. 7～2. 2%. In the range of 103～5150 ng/ml plasma the correlation is good with r= 0. 9999. The detection limit is 52 ng/ml plasma.

关 键 词：高效液相色谱 环丙氟哌酸 血浆 

分 类 号：R969.1[医药卫生—药理学]