反相高效液相色谱荧光检测法测定血浆或肝组织中阿霉素含量  

Determination of Adriamycin in Plasma and Liver Tissue by HPLC with Fluorescence Detector

作　　者：何素梅[1] 魏树礼[1] 吴传斌[1] 王培玉[1] 卢炜[1] 

机构地区：[1]北京医科大学药学院

出　　处：《药物分析杂志》1994年第4期8-11,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：本文报告了反相高效液相色谱荧光检测法测定血浆或肝组织中阿霉素的含量。以正定霉素为内标物，流动相用甲醇－０．０１ｍｏｌ／Ｌ磷酸二氢铵－冰醋酸（７０：３０：０．５）混合液，激发波长４５０ｎｍ，发射波长５３０ｎｒｎ。血浆中药物的方法回收率１０１．５％，相对标准偏差２．７％；肝组织中药物的方法回收率９９．６％，相对标准偏差２．０％。最低检测限６ｎｇ，最低检测浓度２０ｎｇ／ｍｌ。此方法灵敏度高、简单、方便。A high performance liquid chromatography with fluorescence detector for the determination of adriamycin(ADM) in plasma and in liver tissue was reported. Daunorubicin(DM) was used as the internal standard. The mobile phase consisted of 70ml of methanol and 30ml of 0.01mol/L NH_4H_2PO_4 solution containing 0.5ml of acetic acid. Excitation and emission wavelengths were set at 450nm and 530nm. Average recovery and RSD were 101. 5%. 2. 7% in plasma and 99. 6%. 2.0% in liver. respectively. The detection limit of adriamycin is 6ng. The method is simple and highly sensitive.

关 键 词：高效液相色谱 阿霉素 血药浓度 

分 类 号：R969.3[医药卫生—药理学]