多药抗药性的产生与谷胱甘肽的关系  

THECORRELATIONBETWEENMULTIDRUGRESISTANCE ANDGLUTATHIONE(GSH)INEAC,EAC/ADR ANDEAC/ADK(R)CELLLINES

作　　者：王庆端[1] 李国栋[1] 刘梅筠 张予[1] 刘健[1] 张 沐 

机构地区：[1]河南省医学科学研究所药理室

出　　处：《药学学报》1994年第1期20-23,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金

摘　　要：用荧光法对ＥＡＣ及ＥＡＣ／ＡＤＲ细胞内ＧＳＨ含量进行测定，发现ＥＡＣ／ＡＤＲ细胞内ＧＳＨ含量较ＥＡＣ细胞明显增高，分别为１．２５±０．３５及０．３１士０．１２μｇ／１０６细胞；但若对ＥＡＣ／ＡＤＲ细胞株停用ＡＤＲ治疗３６周后，则能使该细胞株部分恢复其敏感性，且ＧＳＨ水平也随之下降。另外，长春新碱、丝裂霉素Ｃ、放线菌素Ｄ及ＶＰ－１６对ＥＡＣ／ＡＤＲ细胞内ＧＳＨ水平均无明显影响。提示：ＧＳＨ水平增高，可能是多药抗药性产生的机理之一。Determination of GSH was performed by modified fluorometric method.Theexperiments showed that the GSH content in resistant cell line(EAC/ADR)was 4 fold higher than thatin sensitive cell line(EAC),being 1. 25士0.35μg/106 cells in EAC/ADR and 0.31士0.12μg/106cells in EAC, When the drug treatment was discontinued for 36 weeks,the sensitivity was partiallyrecovered and the GSH level in EAC / ADR(R)cell parallelly decreased. In addition , vincristine,mitomycin C ,actinomycin D and VP-16 showed no effect on the GSH content in the EAC/ADR cell.

关 键 词：阿霉素 抗药性 谷胱甘肽 抗癌药 

分 类 号：R979.1[医药卫生—药品]