药物对TNF诱导的牛脑微血管平滑肌细胞增值的拮抗作用  

DRUG ANTAGONISM OF TNF INDUCED PROLIFERATION OF BOVINE CEREBROMICROVASCULAR SMOOTH MUSCLE CELLS

作　　者：嵇扬[1] 曾国钱[1] 孙笃新[1] 

机构地区：[1]上海第二军医大学药学院药理教研室,沈阳二0二医院药剂科

出　　处：《药学学报》1994年第2期86-89,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金

摘　　要：研究表明，肿瘤坏死因子（ＴＮＨ）在５０～５０００Ｕ·ｍｌ－１范围内呈剂量依赖性地诱导牛脑微血管平滑肌细胞增殖，ＴＮＦ与该细胞培养２４ｈ时，即可明显刺激细胞增殖，４８ｈ时达最大刺激效应。欧芹素乙（ｉｍｐｅｒａｔｏｒｉｎ，Ｉｍｐ），异欧芹素乙（ｉｓｏ－ｉｍｐｅｒａｔｏｒｉｎ，Ｉｓｉ）在浓度为１０－６～１０－４ｍｏｌ·Ｌ－１时，均可剂量依赖性地拮抗ＴＮＦ诱导该细胞增殖。６－（α，α－二苯基乙酰哌嗪基苯基）－４，５－二氢－５－甲基－３（２Ｈ）哒嗪酮，６－（α－苯基乙酰哌嗪基苯基）－４，５－二氢－５－甲基－３（２Ｈ）哒嗪酮，则只在低浓度（１０－６ｍｏｌ·Ｌ－１）时拮抗ＴＮＦ诱导该细胞的增殖。Effects of tumor necrosis factor(TNF ) on the proliferation of bovine cerebromic-rovascular smooth muscle cells(BCSMC) were investigated.At concentrations from 5 0 to 5 0 0 0U· ml-1, TNF was shown to induce proliferation of cultured BCSMC in a dose-dependent manner. After 24 h incubation of the cells,TNF significantly stimulated the proliferation of BCSMC andreached maximal effects after 48 h incubation , then the effects slightly decreased.At concentrations from l 0-6 to l 0-4mol·L-1,both imperatorin ( Imp) and iso-imperatorin(Isi)were found to antagonize the TNF induced proliferation of BCSMC. Their maximal inhibitoryeffects were 42. 2 and 36.1%at l0-4 mol·L-1 respectively.6-(α,α -diphenylacetylpiperazinly)pheny1-5- methyl-4,5- dihydro-3(2H)-pyridazinone(DMDP ) and 6-(α -phenylacetylpiperazinyl)phenyl- 5- methy-4,5-dihydro-3(2H)- pyridazinone (PMDP) were also found to possess similar effectat lower concentration(10-6mol·L-1),but no significant effect was observed when the drugconcentration was higher.

关 键 词：肿瘤坏死因子 平滑肌 欧芹素乙 

分 类 号：R965[医药卫生—药理学]