高效液相色谱法测定乙吗噻嗪在人的血浆浓度及药代动力学  

DETERMINATION OF ETHMOZINE IN HUMAN PLASMA BY HIGH PERFORMANCE LIOUID CHROMATOGRAPHY AND ITS PHARMACOKINETICS

作　　者：谭力[1] 夏瑶宾 屠锡德[1] 袁倚盛[1] 

机构地区：[1]南京军区南京总医院中心仪器分析科,内蒙古中蒙医院中蒙医研究所,中国药科大学药剂教研室

出　　处：《药学学报》1994年第3期232-236,共5页Acta Pharmaceutica Sinica

摘　　要：建立了反相高效液相色谱法测定人血浆中乙吗噻嗪浓度。色谱柱采用ＳｐｈｅｒｉｓｏｔｂＣ１８柱（２５ｃｍ×４．６ｍｍ，５μｍ），流动相为甲醇—水—三乙胺（７０：３０：０．４，ｐＨ６．５），检测波长２６８ｎｍ。用乙腈沉淀蛋白后，吹干浓缩进样。血药浓度在２０～４０００ｎｇ／ｍｌ范围内呈线性关系，相关系数０．９９９４，血浆最低检测浓度３ｎｇ／ｍｌ。方法回收率９０～１０３％，日内、日间ＲＳＤ２．４～１０．２％。应用该法研究了８名志愿者口服乙吗噻嗪片后的药代动力学，用一室模型拟合，消除相半衰期为１．７５±０．４５ｈ。本法简便、回收率和灵敏度高、重复性好，适于临床药代动力学和药效学的研究。Following detailed study,a rapid and sensitive assay for ethmozine in humanplasma has been developed using reversed phase high performance liquid chromatography(HPLC).Plasma samples were prepared for analysis by addition of internal standard(5-chloro-2-amino-benzophenone )followed by protein preci pitation using acetonitrile.A nalytical column was a C 18 spherisorb. The mobile phase consisted of mathanol water triethylamine(70 :30:0.4,v/v/v,pH6.5).The column effluent was monitored at 268 nm. The calibration curve was linear in the rangefrom 20 ng/ml to 4000 ng/ml with r=0. 9994.The detection limit of this method was 3 ng/ml.Themethed showed good precision and the analytical recovery of ethmozine from plasma was 90～105%;The relative standard deviations for within- day and between- day were 2.4～6;3%and 4.5～ 10.2%respectively.The plasma drug concentration- time course in man after oral administration of 400 mgafter conformed to a 1-compartment open model with a first order absorption phase.Mean T1/2valuewas 1.75+0.45

关 键 词：乙吗噻嗪 高效液相色谱法 药代动力学 

分 类 号：R969.1[医药卫生—药理学]