卡西霉素对血小板在脑微血管内皮细胞粘附的影响及药物的阻断作用  

EFFECTS OF CALCIMYCIN ON PLATELET ADHESION TO CULTURED BoVINE CEREBRAL MICROVASCULAR ENDOTHELIAL CELLS AND ITS ANTAGONISM DRUGS

作　　者：胡晋红[1] 孙笃新[1] 曾国钱[1] 林爱友[1] 芮耀诚[1] 

机构地区：[1]上海第二军医大学药学院药理教研室,上海长海医院药剂科

出　　处：《药学学报》1994年第5期335-339,共5页Acta Pharmaceutica Sinica

摘　　要：体外实验表明ｃａｌｃｉｍｙｃｉｎ０．０１μｍｏｌ·Ｌ－１刺激牛脑微血管内皮细胞２５ｍｉｎ，使兔血小板与脑微血管内皮细胞的粘附率增高１７．１％。ＰＡＦ受体拮抗剂ＷＥＢ２０８６０．１，１．０和１０．０μｕｍｏｌ·Ｌ－对血小板在脑微血管内皮细胞上粘附的抑制率分别为９．０，２２．９和２３．１％。ＤＭＰＰ０．１，１．０，１０．０μｍｏｌ·Ｌ－１及Ｔｅｔ０．１，１．０，１０。Ｏμｍｏｌ·Ｌ－１的抑制率分别为９．７，１５．６，２２．１％和７．８，１５．６及２４．６％，提示ＤＭＰＰ和Ｔｅｔ对脑血管有保护作用。Calcimycin induced rabbit platelet adhesion to cultured bovine cerebral microvascu-lar endothelial cells(CMEC)and its inhibition by triazelodiazepine(WEB 2086),1,5-bis-(3,4-dimethoxyphenyl)-tetrahydro-(4H)-pyran(DMPP)and tetrandrine(Tet )were investigated.TheresuIts showed that calcimycin significantly increased platelet adhesion to CMEC,The plateiet adhesionto CMEC was increased by 17.1%:VS controf after stimulation with calcimycin0.01μmol·L-1 for25 min, WEB 2086 0.1,1.0and l0.0μ mol·L-1 or DMPP 0.1,1. 0 and l0.0μmol·L-1or Tet0.1,1. 0 and l0.0μ mol·L-1 inhibited the calcimycin induced platelet adhesion to CMEC by 9.0,22.9 and 23.1%or9.7, 15.6 and 22.1%or 7.8, 15.6 and 2.6%, respectively.This indicatesthat DMPP and Tet may have perspectives in the prevention af1d treatment of cerebral vascular diseases.

关 键 词：血小板 血管内皮细胞 卡西霉素 

分 类 号：R965[医药卫生—药理学]