多抗甲素抗肿瘤转移作用及其机理  

ANTIMETASTATIC ACTION OF POLYACTIV A ANDITS MECHANISM

作　　者：强文安[1] 刘锦蓉 王浴生[1] 雷幼导[1] 

机构地区：[1]华西医科大学药理教研室,中国医学科学院输血研究所

出　　处：《药学学报》1994年第5期340-345,共6页Acta Pharmaceutica Sinica

摘　　要：多抗甲素（ＰＡＡ）在１００ｍｇ·ｋｇ－１·ｄ－１剂量下，给药１８ｄ，显著抑制Ｂ１６－Ｆ１０黑色素瘤人工肺转移，肺转移结节数由１３７个下降到９５个。同位素参入法测定ＰＡＡ对正常及荷瘤小鼠脾细胞有促增殖作用，并使小鼠脾细胞对ＰＨＡ刺激的反应性不因荷瘤而降低；ＰＡＡ且能增强正常及荷瘤小鼠脾脏ＮＫ细胞活性，拮抗环磷酰胺对ＮＫ细胞活性的抑制作用；ＰＡＡ体外能不同程度地抑制Ｂ１６－Ｆ１０黑色素瘤细胞ＤＮＡ，ＲＮＡ和蛋白质合成。放射免疫测定法表明ＰＡＡ对小鼠血浆ＴＸＡ２／ＰＧＩ２比值影响较小。ＰＡＡ抗肿瘤转移作用主要与促进荷瘤机体抗肿瘤免疫反应和直接抑制肿瘤细胞有关。Polyactin A(PAA)is a home-made immunomodulator,isolated from submerged culture broths of alpha-hemolytic streptococci.The effect of PAA on the metastasis of B16- F1 0melanoma cells in the syngeneic C57BL/6J mice and its antimetastatic mechanism have been studied.The results showed that :PAA inhibited the experimental pulmonary metastasis noules at a dose of 100 mg·kg-1·d-1 for l 8 d.The number of pulmonary metastasis nodules were significantlydecreased from l37 to 95 as compared with those in the control;The plasma coneentration of TXB2 inB16 bearing mice wigher than that in normal mice,After treatment with PAA,a decreased contentof TxB2 and 6-keto-PGF1a was found without change of the ratio TXB2 to 6-keto-pGF1a. The cellularimmunities were evidently decreased in the B16 bearing mice. The restoration of lymphocyte proliferati-on response and auglmentation of the NK cell activity of the splenocytes were found invivo in normalmice and B16 bearing mice after treated with PAA,PAA was also shown to antagonize the suppressingeffect of cycfophosphamide on murine NK cells;PAA at the concentration of i0～5000μg·ml-1 w3sfound to inhibit the biosynthesis of DNA ,RNA and protein in the B16-F1 0 melanoma cells to differentdegrees and the effect was dose-dependent. It is evident that PAA is effective in preventing the pulmonary metastasis of B16- F10 melanomaand.the antimetastatic action may be related not only to promoting the effect the an titumorimmunities ,but also to inhibiting the growth of B16- F10 melanoma cells.

关 键 词：多抗甲素 抗肿瘤转移 药理学 

分 类 号：R965[医药卫生—药理学]