用核磁共振新技术测定川续断皂甙F和H＿1两个新皂甙的结构及光谱规律研究  

APPLICATION OF NEW TECHNIQUES OF NMR IN THE STRUCTURE ELUCIDATION OF TWO NEW SAPONINS, ASPEROSAPONIN F ANDH1,AND SPECTRAL RULES INVOLVED

作　　者：魏峰[1] 楼之岑[1] 刘一民 缪振春[1] 

机构地区：[1]北京医科大学药学院生药学教研室,沈阳药科大学植化教研室,齐齐哈尔市药品检验所,北京军事医学科学院核磁共振实验室

出　　处：《药学学报》1994年第7期511-518,共8页Acta Pharmaceutica Sinica

摘　　要：从川续断（ＤｉｐｓａｃｕｓａｓｐｅｒｏｉｄｅｓＣ．Ｙ．ＣｈｅｎｇｅｔＴ．Ｍ．Ａｉ）根部的乙醇提取物中得到二个新三萜皂甙，命名为川续断皂甙（ａｓｐｅｒｏｓａｐｏｎｉｎｓ）Ｆ和Ｈ１。川续断皂甙Ｆ（Ｉ）是含六个糖的皂甙，川续断皂甙Ｈ１（ＩＩ）是含八个糖的皂甙。运用化学方法及光谱（１ＨＮＭＲ，１３ＣＮＭＲ，１Ｈ－１ＨＣＯＳＹ，一维多重接力ＣＯＳＹ，选择性远程ＤＥＰＴ和三重共振ＮＯＥ差谱）解析，鉴定其结构分别为３－Ｏ－［β－Ｄ－吡喃木糖（１→４）－β－Ｄ－吡喃葡萄糖（１→４）］［α－Ｌ－吡喃鼠李糖（１－→３）］－β－Ｄ－吡喃半乳糖（１→３）－α－Ｌ－吡喃鼠李糖（１→２）－ａ－Ｌ－吡喃阿拉伯糖－常春藤皂甙元（Ｉ）和３－ｏ－［β－Ｄ－吡喃木糖（１→４）－β－Ｄ－吡喃葡萄糖（１→４）］［ａ－Ｌ－吡喃鼠李糖（１→３）］－β－Ｄ－吡喃半乳糖（１→３）－α－Ｌ－吡喃鼠李糖（１→２）－α－Ｌ－吡喃阿拉伯糖－常春藤皂甙元－２８－Ｏ－β－Ｄ－吡喃葡萄糖（１→６）－β－Ｄ－吡喃葡萄糖酯甙（ＩＩ）。并总结出一些光谱方面的变化规律，可用于糖的鉴定，以及构型、糖链的连接顺序和糖与甙元之间的连接位置的说明。Two new triterpenoid saponins,i.e.asperosaponin F(6 sugars)and H1(8sugars), were isolated from the ethanofic extract of the root ofDipsacus asperoides C.Y.Cheng et T.M,Ai.Their structures were identified by chemical and spectral metheds,e.g. 1HNMR,13CNMR,2D1H-1HCOSY,selective long range DEPT,NOEDS, 1D multiple relay COSY. The structures were elucidated as,3-O-[β-D-xylopyranosyl(1→4)-β-D-glucopyranoyl(l→ 4)][ α-L-rhamnopyranosyl(1→3)]-β-D-galactopyranosyl(1→3)-α -L-rhamnopyranosyl(1→2)-α -L-arabinopyranosyl-hederagenin(asperosaponin F,I) and 28-O-β -D-glucopyranosyl(1→6)-β-D-glucopyranoside of F(asperosaponinH1,II).The spectral features for elucidating the structure of saponins containing more than foursugars are also described。

关 键 词：川续断 皂甙 常春藤皂甙元 NMR 

分 类 号：R284.1[医药卫生—中药学]