粉防己碱对胎鼠大脑细胞游离钙含量的影响  

EFFECTS OF TETKANDRINE ON CYTOSOLIC FREE CALCIUM IN FETAL RAT CEREBRAL CELLS

作　　者：车建途[1] 张均田[1] 屈志伟[1] 彭新琦 

机构地区：[1]中国医学科学院,北京协和医科大学药物研究所

出　　处：《药学学报》1994年第7期492-496,共5页Acta Pharmaceutica Sinica

摘　　要：结果表明，粉防己碱（Ｔｅｔ）非常明显地阻断Ｋ＋去极化导致的胎鼠大脑［Ｃａ２＋］ｉ含量的增加。经不同浓度Ｔｅｔ处理的大脑细胞加入ＫＣｌ后，［Ｃａ２＋］ｉ含量仍基本维持在静息状态下水平。对［Ｃａ２＋］ｉ的阻断程度与Ｔｅｔ浓度无关。Ｔｅｔ对Ｌ－谷氨酸（Ｌ－Ｇｌｕ）所致大脑［Ｃａ２＋］ｉ升高亦有明显抑制作用。１０－７ｍｏｌ·Ｌ－１浓度的Ｔｅｔ还降低ＢａｙＫ８６４４引起的［Ｃａ２＋］ｉ上升高。Ｔｅｔ能抑制高Ｋ＋，二氢吡啶类钙激动剂及兴奋性神经递质导致的胎鼠大脑［Ｃａ２＋］ｉ含量增加，提示Ｔｅｔ对神经细胞损伤可能有一定保护作用。Cytosolic free Ca2+─[Ca2+]i was measured in dissociated cerebral cells isolatedfrom fetal rats using the fluorescent indicater Fura-2.Increases in[Ca2+]i occurred rapidly followingexposure of the cells to 50 mmol·L-1 KCl,10-7 mol·L-1 Bay K 8644 and 200 umol·L-1 glu-tamate(Glu ).[ Ca2+]i elevation produced by K+ -depolarization was blocked completely by pretreat-ment with tetrandrine(Tet)of various concentrations(10-9～ 10-7 mol·L-1,final).Tet showedsignificant inhibitory effect on[Ca2+]i increases stimufated by Glu in concentration-dependentmanner.With Tet 10-7 mol·L-1,Bay K 8644-induced increases in[Ca2+]i were reduced_markedly.These results indicate that Tet can block the increases in [Ca2+]i in fetal rat cerebral cellsinduced by Ca2+ agonists,suggesting that this drug may have a protective effect on cerebral cellinJury.

关 键 词：粉防己碱 荧光指示剂 大脑细胞 钙 

分 类 号：R965[医药卫生—药理学]