赛拉嗪对麻醉大鼠海马CA＿1区乙酰胆碱含量的影响  

EFFECT OF XYLAZINE ON ACETYLCHOLINE CONTENT OF THE HIPPOCAMPAL CA1 REGION IN URETHANE ANESTHETIZED RATS RGDm8,

作　　者：丁日高[1] 黄世杰[1] 杨进生[1] 

机构地区：[1]北京军事医学科学院毒物药物研究所

出　　处：《药学学报》1994年第7期481-486,共6页Acta Pharmaceutica Sinica

摘　　要：应用乙酰胆碱选择性微电极技术，观察到小剂量赛拉嗪（２．０和６．０ｍｇ·ｋｇ－１）可显著增加大鼠海马ＣＡ１区ＡＣｈ的含量，而大剂量赛拉嗪（１０．０ｍｇ·ｋｇ－１）及咪唑克生（０．６ｍｇ·ｋｇ－１）则作用相反。咪唑克生虽可明显拮抗赛拉嗪的作用，但海马ＣＡ１区ＡＣｈ的含量仍显著低于正常水平。在去兰斑核的大鼠上，赛拉嗪（２．０和６．０ｍｇ·ｋｇ－１）及咪唑克生（０．６ｍｇ·ｋｇ－１）分别对海马ＣＡ１区ＡＣｈ的含量具有减少和增加作用，且咪唑克生拮抗赛拉嗪的作用后，海马ＣＡ１区ＡＣｈ的含量基本恢复至正常水平。结果提示，赛拉嗪对麻醉大鼠海马ＣＡ１区ＡＣｈ含量呈双相性影响，咪唑可生虽能显著拮抗赛拉嗪的作用，但海马ＣＡ１区ＡＣｈ的含量仍明显低于正常水平，可能分别与赛拉嗪和咪唑克生降低或提高中枢ＮＥ能系统的功能有关。By using acetylcholine selective microelectrode technicque,a biphasic effect ofxylazine on acetylcholine content of the hippocampal CA1 region in urethane anesthetized rats wasobserved as evidenced by the fact that small doses of xylazine2.0 and 6.0 mg·kg-1)significantlyincreased,while higher dose(10.0 mg·kg-1 )decreased the acetylcholine content.Though idazoxan(0.6 mg·kg-1 ) significantly antagonized the effect of xylazine when used in combination,theacetylcholine content in the hippocampal CA1 region of urethane anesthetized rats was still much lowerthan that of the control group.In rats whose nucleus locus coeruleus was chemically lesioned,xylazine(2.0 and 6.0 mg·kg-1)significantly decreased the acetylcholine content,and idazoxan(0.6mg· kg-1),which increased the acetylcholine content in the hippocampal CA1 region when usedalone,completely antagonized the effect of xylazine,These results suggest that the biphasic effect ofxylazine and the lower than normal acetylcholine content oherved when idazoxan was used toantagonize xylazine induced changes in acetylcholine content of the hippocampal CA1 region inurethane anesthetized rats might be related to the effects of xylazine and idazoxan on the centralnoradrenergic neurotransmitter system.

关 键 词：赛拉嗪 兰斑核 海马 乙酰胆碱 

分 类 号：R965[医药卫生—药理学]