毛细管气相色谱－电子捕获法测定人血清中5－单硝酸异山梨醇酯的浓度和药代动力学参数  

GAS CHROMATOGRAPHIC METHOD WITH ECDETECTION TO DETERMINE ISOSORBIDE-5-MONONITRATE(IS-5-MN)IN HUMAN SERUM AND ITS PHARMACOKINETIC PAPAMETERS

作　　者：夏桂珠[1] 傅贻柯[1] 董善年[1] 李然[1] 

机构地区：[1]北京医科大学分析化学教研室,北京医科大学应用药物研究所

出　　处：《药学学报》1994年第8期634-638,共5页Acta Pharmaceutica Sinica

摘　　要：毛细管气相色谱－电子捕获法测定人血清中５－单硝酸异山梨醇酯的浓度和药代动力学参数夏桂珠，傅贻柯，董善年，李然（北京医科大学分析化学教研室，北京医科大学应用药物研究所１０００８３）５－单硝酸异山梨醇酯（ｉｓｏｓｏｒｂｉｄｅ－５－ｍｏｎｏｎｉｔｒａｔｅ，...A simple, sensitive and precise capillary GC-ECD method was developed for thedetermination of isosorbide-5-mononitrate in human serum,Pharmacokinetic parameters of the drugwas obtained from the human serum level-time curve measured. Serum samples were extracted with a mixture of ethyl ether-ethyl acetate(4:1), the upperphase was collected and evaporated to about 100μl under a gentle nitrogen stream,Isosorbide dinitratewas used as internal standard.With a human serum sample size of 200μl ,the detection limit of IS-5-MN was found to be about 5 ng/ml,and the athelute recovery from 74%to 85%. The within-dayand between-day relative standard deviation were less than 7%and 9%,respectively. This method was applied to the pharmacokinetic studies of IS-5-MN tablets froth two differentsouroes.Two sets of t1/2(Ke),Tmax and AUC values obtained from 8 volunteers were tested statisti-cally and no significant difference was found.

关 键 词：单硝酸 异山梨醇酯 气相色谱 

分 类 号：R969.1[医药卫生—药理学]