流式细胞仪对系统性红斑狼疮患者CD8^+T淋巴细胞亚群的检测  

Assay of CD8^+ T Lymphocytes in Systemic Lupus Erythmatosus Patients by Flow Cytometery

作　　者：赵宏丽[1] 孙晓慧[1] 赵俊芳[1] 李孟娟[1] 丁素光[1] 

机构地区：[1]天津市长征医院,天津300021

出　　处：《中国中西医结合皮肤性病学杂志》2005年第1期7-9,共3页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

摘　　要：目的探讨CD+8T淋巴细胞对系统性红斑狼疮的作用机制。方法用流氏细胞仪直接免疫荧光法检测SLE患者外周血CD3+、CD4+、CD8+、CD28+T淋巴细胞的百分率。结果活动期SLE患者CD3+细胞、CD8+细胞百分率明显高于对照组和缓解期患者,CD4+/CD8+比值亦明显降低(P<0.05),CD4+细胞百分率比较无统计意义。在CD8+细胞中,CD8+CD28-细胞的百分率明显高于对照组和稳定期患者(P<0.05)。稳定期患者,CD3+细胞、CD4+细胞、CD8+细胞、CD4+/CD8+比值与对照组比较均无显著性差异(P>0.05)。在CD8+细胞中,CD8+CD28-细胞所占百分率与对照组比较无统计意义(P>0.05)。结论SLE活动期患者虽然CD8+细胞增多,但大多数是CD8+CD28-细胞,失去细胞毒性作用。相反,与活动期患者相比,稳定期患者体内生成CD8+CD28+淋巴细胞。CD8+CD28-T细胞在SLE的发病机制中起着一定的作用,且患者体内的CD8+T抑制淋巴细胞的变化是可逆的。Objective To investigate the pathogenesis of T lymphocytes in systemic lupus erythmatosus(SLE). Methods Direct immunofluorescence is used to assay the percentage of CD3+,CD4+,CD8+ and CD28+ T lymphocytes by flow cytometry. Results The percentage of CD3+ T lymphocytes and CD8+ T lymphocytes in active stage is higher than that in remissive stage and control. The ratio of CD4+/CD8+ decreases in active stage. The percentage of CD8+CD28- T lymphocytes in CD8+ T lymphocytes increases in active stage. In remissive stage, the percentage of CD3+,CD4+,CD8+ T lymphocytes, the ratio of CD4+/CD8+ and the percentages of CD8+CD28- T lymphocytes in CD8+ T lymphocytes have no significant difference. Conclusion CD8+ T lymphocytes increases in active stage of SLE, but most of them are CD8+CD28- T lymphocytes. They lost inhibitive activity. Whereas the body of patients in remissive stage can produce CD8+CD28+ T lymphocytes which have inhibitive activity. CD8+CD28- T lymphocytes play important role in the pathogenesis of SLE, and the change of them can be reverse.

关 键 词：患者 CD8^+细胞 CD8^+CD28^+ 对照组 SLE CD3^+ 系统性红斑狼疮 百分率 结论 意义 

分 类 号：R593.241[医药卫生—内科学] R512[医药卫生—临床医学]