新型聚β-羟基丁酯作为血管组织工程支架材料的实验研究  

Experimental studies on a neotype polyhydroxybutyrate as scaffold for vascular tissue engineering

作　　者：艾卫兵[1] 艾玉峰[1] 熊猛[1] 田晋洪[1] 易成刚[1] 伍锦华[1] 张琳西[1] 杨清芳[2] 董兆麟[3] 

机构地区：[1]第四军医大学西京医院整形外科,陕西省西安市710032 [2]西北工业大学化学工程系 [3]西北大学生物工程系

出　　处：《中国美容医学》2005年第2期140-142,i001,共4页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金资助项目(30070777)

摘　　要：目的:探讨经改性处理后的可降解生物材料聚β-羟基丁酯(PHB)作为血管组织工程支架材料与血管平滑肌细胞的细胞相容性。方法:采用体外培养的兔血管平滑肌细胞接种在经胶原包埋处理后的管型PHB支架材料上,用相差显微镜和扫描电镜观察细胞的粘附和生长情况,并行HE染色观察。结果:兔血管平滑肌细胞在改性后的管型PHB支架材料上粘附生长良好,扫描电镜下可见细胞生长融合成片状,HE染色示细胞在PHB材料上生长良好。结论:改性后的聚β-羟基丁酯材料与兔血管平滑肌细胞有较好的生物相容性。Objective To study the cellular biocompatibility of polyhydroxybutyrate(PHB) which acted as scaffold for vascular tissue engineering combined with vascular smooth muscle cells(VSMCs). Methods VSMCs collected from rabbit were cultured in vitro, and were seeded in PHB embedded with collagen. Cell adhesion, spreading and growth were observed by phase contrast microscope,SEM and HE staining. Results VSMCs could attach, spread and proliferate well in PHB embedded with collagen. Conclusion PHB embedded with collagen has good biocompatibility with VSMCs

关 键 词：血管组织工程 聚β-羟基丁酯 血管平滑肌细胞 细胞相容性 

分 类 号：R619.6[医药卫生—外科学]